Phaseolus vulgaris is nodulated in northern Spain by Rhizobium leguminosarum strains harboring two nodC alleles present in American Rhizobium etli strains: biogeographical and evolutionary implications

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Abstract:

In this study a collection of rhizobial strains were isolated from effective nodules of Phaseolus vulgaris in a wide region of northern Spain, which is the major producer region of this legume in Spain. The analysis of their core genes, rrs, atpD, and recA, and the 16S-23S intergenic spacer showed that all isolates belong to the phylogenetic group of Rhizobium leguminosarum and some of them were identical to those of strains nodulating Vicia or Trifolium. None of the isolates was identified as Rhizobium etli; however, all of them carry the nodC alleles α and  harboured by American strains of this species. These alleles were also found in strains nodulating P. vulgaris in southern Spain identified as R. etli. These results suggest that R. etli was carried from America to Spain with common bean seeds, but that they could have found difficulties persisting in the soils of northern Spain, probably because of the climatic conditions. The symbiotic genes of this species could have been transferred, after the arrival of P. vulgaris, to strains of R. leguminosarum already present in northern Spanish soils.

Dans cette étude, une collection de souches de rhizobiums a été isolée de nodules actifs de Phaseolus vulgaris dans une vaste région du Nord de l’Espagne, principale productrice de cette légumineuse dans cette région. L’analyse des gènes centraux rrs, atpD et recA, et de l’espaceur intergénique 16S-23S, a démontré que tous les isolats appartenaient au groupe phylogénique de Rhizobium leguminosarum et que quelques uns d’entre eux étaient identiques aux souches formant des nodules sur Vicia ou Triflorum. Aucun isolat n’a été identifié à Rhizobium etli, mais tous possédaient les allèles α et  de nodC retrouvées dans les souches américaines de cette espèce. Ces allèles ont aussi été retrouvés dans les souches formant des nodules sur P. vulgaris du sud de l’Espagne, identifiées à R. etli. Ces résultats suggèrent donc que R. etli a été apporté de l’Amérique vers l’Espagne par des graines de fèves communes, mais qu’il a eu de la difficulté à persister dans les sols du nord de l’Espagne, probablement à cause des conditions climatiques. Les gènes symbiotiques de cette espèce pourraient avoir été transférés après l’arrivée de P. vulgaris vers les souches de R. leguminosarum déjà présentes dans les sols du nord de l’Espagne.

Document Type: Research Article

Publication date: August 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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