Genomic analysis of two ORF2 segments of new porcine astrovirus isolates and their close relationship with human astroviruses

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Abstract:

Porcine astrovirus (PAstV) has been poorly studied and has been associated mainly with gastroenteritis. Computational analysis has revealed the close relationship of PAstV with astroviruses of humans (HAstV) and cats (FAstV). In this study, 105 and 171 stool specimens were collected from piglets and children under 5 years of age, respectively, in different Colombian regions during a 1-year period. The stool samples were examined for astroviruses by ELISA and RT-PCR; 23.8% and 4% were found to be positive for PAstV and HAstV, respectively. Additional sequence analysis with partial sequences obtained from ORF2 identified at least 2 probable groups of PAstVs and possible recombination events between porcine and human astroviruses. This study provides preliminary evidence of the high presence of PAstVs in pigs and proposes the existence of multiple PAstV types that are evolutionarily closely related to HAstVs.

Les astrovirus porcins (PAstV), qui ont été peu étudiés, sont associés principalement à la gastroentérite. Une analyse bio-informatique a révélé qu’ils sont étroitement liés aux astrovirus humains (HAstV) et félins (FAstV). Dans cette étude, 105 et 171 échantillons de selles ont été respectivement recueillis de porcelets et d’enfants de moins de 5 ans, dans différentes régions de la Colombie et ce, pendant 1 an. La présence d’astrovirus dans les échantillons de selles a été examinée par ELISA et RT-PCR. Les PAstV et HAstV ont été respectivement trouvés dans 23,8 % et 4 % des échantillons de selles. Des analyses supplémentaires de séquences partielles obtenues du cadre de lecture ouvert 2 (ORF2) ont permis d’identifier au moins 2 groupes probables de PAstV, et de mettre en évidence une recombinaison possible entre les astrovirus porcins et humains. Cette étude donne des preuves préliminaires de la présence élevée de PAstV chez le porc, et elle propose qu’il existe de multiples types de PAstV étroitement liés d’un point de vue évolutif aux HAstV

Document Type: Research Article

Publication date: July 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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