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Changes in the structure and diversity of bacterial communities during the process of adaptation to organic wastewater

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Abstract:

To explore changes in the structure and diversity of activated sludge-derived microbial communities during adaptation to gradual increases in the concentration of wastewater, RAPD-PCR and the combination of PCR amplification of 16S rRNA genes with denaturing gradient gel electrophoresis (DGGE) analysis were used. In bacterial communities exposed to 0%, 5%, 10%, 20%, or 40% wastewater, there were 27, 25, 18, 17 and 16 bands, respectively, based on DGGE data, while there were 69, 83, 97, 86, and 88 bands, respectively, based on RAPD data. The community similarity index among bacterial communities during the process of adaptation to different concentrations of wastewater was different based on DGGE and RAPD data. Based on DGGE and RAPD profiles, the Shannon-Weiner and Simpson’s diversity indices decreased sharply upon exposure to 10% wastewater, indicating that 10% wastewater might be a critical point at which the growth of bacteria could be significantly inhibited and the genotypic diversity could change. This indicated that changes in structure and diversity might have an inhibitory effect on the toxicity of organic matter and that selection and adaptation could play important roles in the changes.

Un protocole de PCR-RAPD et une combinaison d’amplification par PCR des gènes d’ARNr 16 S et d’électrophorèse sur gel en gradient dénaturant (DGGE) ont été utilisés afin d’explorer les changements dans la structure et la diversité des communautés microbiennes dérivées des boues lors d’une adaptation à des augmentations graduelles de la concentration d’eaux usées. Chez les communautés bactériennes exposées à 0 %, 5 %, 10 %, 20 % ou 40 % d’eaux usées, la DGGE permettait de détecter 27, 25, 18, 17 et 16 bandes respectivement, alors que les données obtenues par RAPD révélaient la présence de 69, 83, 97, 86 et 88 bandes respectivement. Les indices de similarité de communautés de ces communautés bactériennes différaient durant le processus d’adaptation aux eaux usées à différentes concentrations, selon les données en RAPD ou en DGGE. Les indices de diversité de Shannon-Weiner et de Simpson diminuaient abruptement à partir d’une exposition à 10 % d’eaux usées, selon les profils en DGGE et en RAPD, indiquant qu’une concentration de 10 % en eaux usées constituerait un seuil à partir duquel la croissance bactérienne serait significativement inhibée et la diversité bactérienne changée durant le processus d’adaptation. Ceci a indiqué que les changements dans la structure et la diversité pourraient résulter d’un effet inhibiteur causé par la toxicité des matières organiques, et que la sélection et l’adaptation ont joué un rôle important dans ces changements.

Document Type: Research Article

Publication date: April 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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