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Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance

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Abstract:

A Candida-specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.

Un test multiplex à onze sondes spécifique pour détecter Candida, basé sur la technologie Luminex, a été utilisé pour identifier rapidement des isolats de Candida sp 1182 qui ont été obtenus dans le cadre d’un programme de dépistage populationnel présentement en cours. Tous les isolats de Candida ont été préalablement identifiés par une combinaison de méthodes, notamment par des analyses de phénotypes et de séquences. Les résultats ont montré que le test Luminex constitue une alternative intéressante aux méthodes de référence car il est rapide, il conduit à une identification correcte de l’espèce et il est convivial.

Document Type: Research Article

Publication date: April 1, 2010

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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