Chemical composition of lipopolysaccharides isolated from various endophytic nitrogen-fixing bacteria of the genus Herbaspirillum

Authors: Serrato, R. V.; Sassaki, G. L.; Cruz, L. M.; Carlson, R. W.; Muszyński, A.; Monteiro, R. A.; Pedrosa, F. O.; Souza, E. M.; Iacomini, M.

Source: Canadian Journal of Microbiology, Volume 56, Number 4, April 2010 , pp. 342-347(6)

Publisher: NRC Research Press

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Abstract:

Bacteria from the genus Herbaspirillum are endophytes responsible for nitrogen fixation in gramineous plants of economic importance such as maize, sugarcane, sorghum, rice, and wheat. Some species are known to produce plant growth substances. In contrast, Herbaspirillum rubrisubalbicans strains are known to be mild plant pathogens. The molecular communication between the plant and the microbes might involve lipopolysaccharides present in the outer membrane of these gram-negative bacteria. Phenol-water extraction was used to obtain lipopolysaccharides from 7 strains of Herbaspirillum seropedicae (SmR1, Z67, Z78, ZA95, and M2) and H. rubrisubalbicans (M1 and M4). The electrophoretic profiles and chemical composition of the lipopolysaccharides obtained in the phenol and aqueous extracts were shown herein.

Les bactéries du genre Herbaspirillum sont des endophytes responsables de la fixation d’azote chez les graminées d’importance économique telles le maïs, la canne à sucre, le sorgho, le riz et le blé. Quelques espèces sont connues pour produire des substances favorisant la croissance végétale. Au contraire, les souches de Herbaspirillum rubrisubalbicans sont des pathogènes végétaux bénins. La communication moléculaire entre les végétaux et les microbes peut impliquer des lipopolysaccharides présents sur le membrane externe de ces bactéries négatives à gram. Une extraction phénol-eau a été utilisée pour obtenir les lipopolysaccharides de 7 souches de Herbaspirillum seropedicae (SmR1, Z67, Z78, ZA95 et M2) et de H. rubrisubalbicans (M1 et M4). Les profils en électrophorèse et la composition chimique des LPS obtenus des extraits phénoliques et aqueux sont présentés ici.

Document Type: Research Article

Publication date: April 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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