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Comparison of the automated Phoenix with the Vitek 2 for the identification of Streptococcus pneumoniae

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Abstract:

Rapid and accurate identification of Streptococcus pneumoniae is a critical component in the optimal management of infected patients. The performance of the BD Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, Md.) was evaluated for identification of S. pneumoniae (n = 311) and was compared to the Vitek 2 (bioMérieux, Marcy l’Étoile, France). Strains with discordant identification between methods were resolved with 16S rRNA gene sequencing as the gold standard. The Phoenix and the Vitek 2 correctly identified 96.8% (n = 301) and 95.2% (n = 296) of S. pneumoniae strains, respectively. Overall, there was no statistically significant difference in the performance of the 2 automated systems for the identification of S. pneumoniae in this study. The Vitek 2 mean time-to-results for all streptococcal identification was 1.5 h faster than that for the Phoenix. We conclude that the automated Phoenix and the Vitek 2 systems are comparable in their ability to identify S. pneumoniae and are preferable to the use of routine biochemical assays, which have delayed time-to-results and are not dependably accurate.

L’identification rapide et fiable de Streptococcus pneumoniae est critique à la prise en charge optimale des patients infectés. La performance du système automatisé BD Phoenix (BD Diagnostic Systems, Sparks, Md.) a été évaluée dans l’identification de S. pneumoniae (n = 311), et elle a été comparée à celle du système Vitek 2 (bioMérieux, Marcy l’Étoile, France). Les souches dont les indentifications par les deux méthodes étaient discordantes ont été résolues par séquençage de ARNr 16S comme utilisé comme étalon-or. Phoenix et Vitek 2 ont correctement identifié 96,8 % (n = 301) et 95,2 % (n = 296) des souches de S. pneumoniae, respectivement. Globalement, il n’y avait pas de différence significative dans la performance des 2 systèmes automatisés pour identifier S. pneumoniae dans cette étude. Le temps moyen d’obtention des résultats d’identification de tous les streptocoques du Vitek 2 était 1,5 h plus rapide que celui du Phoenix. Nous concluons que les systèmes automatisés Phoenix et Vitek 2 sont comparables dans leur capacité à identifier S. pneumoniae et qu’ils sont préférables à l’utilisation des essais biochimiques de routine, qui sont plus longs sans être plus fiables pour autant.

Document Type: Research Article

Publication date: April 1, 2010

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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