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Evaluation of the role of enzymatic and nonenzymatic antioxidant systems in the radiation resistance of Deinococcus

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Abstract:

Antioxidant enzymes and antioxidant metabolites appear to have different roles in the oxidative stress resistance responses of radiation-resistant bacteria belonging to the Deinococcus-Thermus group. Twelve distinct strains belonging to 7 Deinococcus species were characterized for their responses to hydrogen peroxide, ciprofloxacin, and ionizing radiation. The levels of catalase and peroxidase activities in these strains showed a positive correlation with resistance to hydrogen peroxide and ciprofloxacin. However, the levels of these enzymes and carotenoids did not appear to contribute significantly to radiation resistance. Our findings support the idea that enzymatic defense systems are not sufficient to account for the extreme radiation resistance of Deinococcus species. Consistent with previously published reports, the Deinococcus strains had high intracellular manganese/iron ratios. No significant correlation was found between intracellular manganese/iron ratios and radiation resistance within different Deinococcus species, suggesting that other components are involved in conferring radiation resistance.

Les enzymes antioxydantes et les métabolites antioxydants semblent jouer différents rôles dans la résistance au stress oxydatif chez les bactéries appartenant au groupe Deinococcus-Thermus. Douze souches distinctes appartenant à 7 espèces de Deinococcus ont été caractérisées quant à leurs réponses au peroxyde d’hydrogène, au cyprofloxacin et aux radiations ionisantes. Les niveaux d’activité de la catalase et de la peroxydase chez ces souches étaient positivement corrélés avec la résistance au peroxyde d’hydrogène et au cyprofloxacin. Cependant, les niveaux d’enzymes et de caroténoïdes ne semblaient pas contribuer significativement à la résistance aux radiations. Nous résultats appuient l’idée que les systèmes de défense enzymatiques sont insuffisants pour expliquer l’extrême résistance aux radiations de Deinococcus. Conformément aux précédents rapports publiés, les souches de Deinococcus possédaient des ratios élevés manganèse/fer intracellulaires. Aucune différence significative n’a été trouvée entre les ratios manganèse/fer intracellulaires et la résistance aux radiations des différentes espèces de Deinococcus, ce qui suggère que d’autres composantes sont impliquées dans la résistance aux radiations.

Document Type: Research Article

Publication date: March 1, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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