Mode of action of thuricin S, a new class IId bacteriocin from Bacillus thuringiensis

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Abstract:

Different methods were used to elucidate the mode of action of thuricin S, a new class IId bacteriocin produced by Bacillus thuringiensis subsp. entomocidus HD198. According to cell viability tests, thuricin S was shown to exert a bactericidal effect on the sensitive cells of Bacillus thuringiensis subsp. darmastadiensis 10T. The use of the fluorescent probe 3,3′-dipropylthiadicarbocyanine iodide as an indicator proved that thuricin S interacts with the cytoplasmic membrane to dissipate the transmembrane potential. It was also demonstrated that thuricin S acts as a pore-forming bacteriocin, since it allows the nonpermeable stain propidium iodide to enter the cells. The loss of membrane integrity and the morphological changes in sensitive cells were visualized by scanning electron microscopy.

Différentes méthodes ont été utilisées pour élucider le mode d’action de la thuricine S, une nouvelle bactériocine de la classe IId produite par Bacillus thuringiensis subsp. entomocidus HD198. Les différents tests de viabilité cellulaire, ont montré que la thuricine S exerce une action bactéricide sur les cellules sensibles de Bacillus thuringiensis subsp. darmastadiensis 10T. L’utilisation de la sonde fluorescente 3,3′-dipropylthiadicarbocyanine iodide a montré que la thuricine S interagit avec la membrane cytoplasmique et aboli le potentiel transmembranaire. De même, il a été démontré que la thuricine S agit en provoquant la formation des pores puisqu’elle a permis à l’iodure de propidium de pénétrer dans les cellules. La perte de l’intégrité membranaire ainsi que les changements morphologiques des cellules sensibles ont été visualisés par microscopie électronique à balayage.

Document Type: Research Article

Publication date: February 2, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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