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Characterization and expression of a novel holotype insecticidal crystal protein gene from native Bacillus thuringiensis BM59-2

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Abstract:

We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 µg/mL (95% confidence: 0.740-3.499 µg/mL).

Nous avons caractérisé un nouveau gène holotype cry (cry52Ba1) contenu dans l’isolat BM59-2 de Bacillus thuringiensis, originaire de Chine, qui exprime une protéine d’environ 80 kDa. Dans cette étude, le gène cry52Ba1 de pleine longueur a été cloné à partir de cette souche et la séquence du gène a été déterminée et analysée. De plus, cry52Ba1 a été exprimé chez Escherichia coli BL21(DE)pLysS et le résultat de la mesure de l’activité insecticide a montré que la protéine Cry52Ba1 possédait une forte activité larvicide envers Aedes aegypti (Diptère), avec une concentration létale à 50 % (CL50) de 1.526 µg/mL (95 % de confiance: 0.740-3.499 µg/mL).

Document Type: Research Article

Publication date: February 2, 2010

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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