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Proteomic analysis of Trichoderma atroviride mycelia stressed by organophosphate pesticide dichlorvos

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Abstract:

The proteomic approach is a powerful tool to study microbial response to environmental stress. To evaluate the responses of Trichoderma spp. to the organophosphate pesticide dichlorvos, mycelia of Trichoderma atroviride T23 were exposed to dichlorvos at concentrations of 0, 100, 300, 500, and 1000 µg/mL, respectively. Changes in protein expression were investigated using two-dimensional sodium dodecyl sulfate - polyacrylamide gel electrophoresis. Sixteen protein spots were differentially expressed. They were identified by MALDI-TOF/TOF MS and were found to be linked to energy metabolism, transport, signal transduction, and stress tolerance. Among stress-related proteins, glutathione peroxidase-like protein (GPX), 1,4-benzoquinone reductase, and HEX1 were upregulated by and cyclophilin A induced by 1000 µg/mL dichlorvos when compared with the control. These proteins were considered to be associated with fungal adaptation to adverse conditions. The results will help us to understand molecular mechanisms through which Trichoderma responds to organophosphate pesticides.

L’approche par protéomique constitue un outil puissant pour étudier la réponse microbienne à un stress environnemental. Afin d’évaluer la réponse de Trichoderma spp. au pesticide dichlorvos, un organophosphate, des mycéliums de Trichoderma atroviride T23 ont été exposés au dichlorvos à des concentrations de 0, 100, 300, 500 et 1000 µg/mL respectivement. Les changements dans l’expression des protéines ont été examinés par électrophorèse sur gel de polyacrylamide - sodium dodécyl sulfate en deux dimensions (SDS-PAGE 2D). Seize taches correspondant à des protéines exprimées de façon différentielle ont été observées. Elles ont été identifiées par MALDI-TOF/TOF MS et se sont avérées liées au métabolisme énergétique, au transport, à la transduction de signaux et à la tolérance au stress. Parmi les protéines associées au stress, la glutathion peroxidase GPX, la 1,4-benzoquinone réductase et HEX1 étaient régulées à la hausse, et la cyclophiline A était induite par 1000 µg/mL de dichlorvos, comparativement au contrôle. Ces protéines ont été considérées associées à l’adaptation des champignons à des conditions défavorables. Ces résultats vont nous aider à comprendre les mécanismes moléculaires par lesquels Trichoderma réagit aux organophosphates.

Document Type: Research Article

Publication date: 2010-02-02

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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