Short hairpin RNA-mediated inhibition of measles virus replication in vitro

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Rab9 has been identified as a key component for the replication of measles virus (MV). In this study, gene-specific shRNAs were developed to suppress the replication of MV in culture cells by silencing the expression of Rab9 GTPase gene. Rab9 GTPase gene-specific shRNAs were designed and cloned into the expression vector of pSUPER.neo+EGFP. Vero-E6 cells were transfected with the recombinant plasmid via liposome and then infected with MV. The expression of Rab9 GTPase mRNA and protein were assayed by RT-PCR and Western blotting, respectively. ShRNA-mediated inhibition of MV replication was further evaluated by detecting the titer of MV. The results showed that the expression of Rab9 GTPase was dramatically and stably downregulated by the generated shRNAs targeting Rab9 GTPase gene, which contribute to the inhibition of MV replication, indicating these shRNAs could be potentially developed into therapeutic agents for the treatment of MV infection in the future.

Rab9 est une composante clé de la réplication du virus de la rougeole (VR). Dans cette étude, de petits ARN hétérogènes (ARNsh) ont été développés pour supprimer la réplication du VR dans les cellules en culture en inhibant l’expression du gène de la GTPase Rab9. Les ARNsh spécifiques au gène de la GTPase Rab9 ont été conçus et clonés dans le vecteur d’expression pSUPER.neo+EGFP. Les cellules Vero-E6 ont été transfectées avec le plasmide recombinant à l’aide de liposomes et ont ensuite été infectées par le VR. L’expression de la GTPase Rab9 et de son ARNm ont été mesurées par buvardage Western et par RT-PCR respectivement. L’inhibition de la réplication du VR causée par l’ARNsh a été davantage mesurée par le titrage du VR. Les résultats ont montré que l’expression de la GTPase Rab9 était diminuée de façon importante et stable par les ARNsh qui ciblent la GTPase Rab9, lesquels contribuent à l’inhibition de la réplication du VR, indiquant que ces ARNsh pourraient potentiellement être développés en agents thérapeutiques dans le traitement de l’infection par le VR dans l’avenir.

Document Type: Research Article

Publication date: January 1, 2010

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more