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Transformation of Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 by CaCl2 method

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Abstract:

The CaCl2 method, commonly used for transformation of Escherichia coli, was modified and used to develop a simpler and easier transformation method for Rhizobia sp. Two species of Rhizobia, Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620, were transformed with the 13.2 kb binary vector pGA482. At an optical density of 0.4, the transformation efficiencies in Sinorhizobium meliloti MTCC 100 and Mesorhizobium ciceri TAL 620 were 104 and 103, respectively. Competent cells of Sinorhizobium meliloti MTCC 100 were prepared at different growth intervals and transformed by the same vector. A maximum transformation efficiency of 104 was achieved at an optical density of 0.5.

La méthode du CaCl­2 couramment employée pour transformer E. coli a été modifiée et utilisée pour développer une méthode de transformation plus simple et plus facile pourRhizobia spp. Deux espèces de Rhizobia, Sinorhizobium meliloti MTCC 100 et Mesorhizobium ciceri TAL620 ont été transformées avec le vecteur binaire de 13.2 kb pGA482. À une densité optique de 0.4, les efficacités de transformation de Sinorhizobium meliloti MTCC 100 et Mesorhizobium ciceri TAL620 étaient de 104 et 103 respectivement. Des cellules compétentes de Sinorhizobium meliloti MTCC 100 ont été préparées à différents intervalles de croissance et ont été transformées avec le même vecteur. Une efficacité maximale de transformation de 104 a été obtenue à une densité optique de 0.5.

Document Type: Research Article

Publication date: 2010-01-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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