If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Comparison of antifungal efficacies of moxifloxacin, liposomal amphotericin B, and combination treatment in experimental Candida albicans endophthalmitis in rabbits

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

The goal of this study was to compare in vitro and in vivo efficacy of moxifloxacin and liposomal amphotericin B (Amp-B) monotherapies and combination treatment against Candida albicans in an exogenous endophthalmitis model in rabbit eyes. Microplate dilution tests and checkerboard analysis were performed to detect in vitro efficacies. Endophthalmitis was induced by intravitreal injection of C. albicans in 40 rabbit eyes with simultaneous intravitreal drug injection according to prophylactic treatment groups. Group 1 (control group) received 0.1 mL of balanced salt solution, group 2 (moxi group) 100 µg moxifloxacin/0.1 mL, group 3 (Amp-B group) 10 µg liposomal Amp-B/0.1 mL, and group 4 (combi group) both 100 µg moxifloxacin/0.05 mL and 10 µg liposomal Amp-B/0.05 mL intravitreally. Clinical examination, quantitative analysis of microorganisms, and histopathologic examination were performed as in vivo studies. The minimum inhibitory concentration of liposomal Amp-B against C. albicans was found to be 1 µg/mL. Moxifloxacin showed no inhibition of in vitro C. albicans growth. The minimum inhibitory concentration values of liposomal Amp-B for C. albicans were reduced two- to eightfold with increasing concentrations of moxifloxacin in vitro. In vivo, there was no C. albicans growth in the combi group (zero of eight eyes), whereas three eyes (37.5%) showed growth in the Amp-B group. Vitreous inflammation, retinal detachment, focal retinal necrosis, and outer nuclear layer loss were found to be lower in the moxi group compared with the control group. Ganglion cell and inner nuclear layer loss was observed in all eyes (100%) in both the moxi and combi groups, whereas only in 25% (two of eight eyes) in the Amp-B group. Moxifloxacin strongly augments the efficacy of liposomal Amp-B against C. albicans in vitro, although it has no in vitro antifungal activity when used alone. It is interesting that we found a synergistic effect for in vitro tests but failed to demonstrate it in vivo. When 100 µg moxifloxacin/0.1 mL is given intravitreally, it has some toxic effects that are limited to the inner retinal layers.

Comparer l’efficacité in vitro et in vivo de monothérapies ou de traitements combinés à la moxifloxacine et à l’amphotéricine B (Amp-B) sous forme liposomale envers Candida albicans dans un modèle d’endophtalmie exogène chez le lapin. Des tests de dilution en microplaques et une analyse en damier ont été réalisés pour détecter l’efficacité des traitements in vitro. L’endophtalmie a été induite par injection intravitréenne de C. albicans dans 40 globes oculaires de lapins, simultanément à une injection intravitréenne de médicament, selon les groupes de traitement prophylactique. Le groupe 1 (groupe contrôle) a reçu 0.1 mL de solution saline tamponnée; le groupe 2 (groupe moxi), 100 µg/0.1 mL de moxifloxacine; le groupe 3 (groupe Amp-B), 10 µg/0.1 mL d’Amp-B liposomale; le groupe 4 (groupe combi), 100 µg/0.05 mL de moxifloxacine et 10 µg/0.05 mL d’Amp-B liposomale dans le vitré. Les études in vivo consistaient en un examen clinique, une analyse quantitative des microorganismes et un examen histopathologique. La CIM de l’Amp-B liposomale envers C. albicans était de 1 µg/mL. La moxifloxacine n’inhibait pas la croissance de C. albicans in vitro. Les valeurs de CIM de l’Amp-B liposomale envers C. albicans diminuaient de 2 à 8 fois en fonction des concentrations croissantes de moxifloxacine in vitro. In vivo: aucune croissance de C. albicans n’a été observée dans le groupe combi (0/8 globes oculaires) alors qu’une certaine croissance était observée dans le groupe Amp-B (3 globes oculaires; 37.5%). L’inflammation du vitré, le décollement de la rétine, la nécrose rétinienne focale et la perte de la couche nucléaire externe étaient moindres dans le groupe moxi comparativement au groupe contrôle. La perte de cellules ganglionnaires et de la couche nucléaire interne a été observée dans 100% des globes oculaires chez les groupes moxi et combi, mais dans 25% seulement des globes oculaires du groupe Amp-B. La moxifloxacine a fortement augmenté l’efficacité de l’Amp-B liposomale envers C. albicans in vitro, quoiqu’elle n’exerçait aucune activité antifongique in vitro lorsque utilisée seule. Il est intéressant de constater que nous avons trouvé un effet synergique lors des tests in vitro mais aucun in vivo. La moxifloxacine 100 µg/0.1 mL administrée dans le vitré exerçait un certain effet toxique limité aux couches rétiniennes internes.

Document Type: Research Article

Publication date: January 1, 2010

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more