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Expression of the atf1+ gene is upregulated in fission yeast under nitrosative and nutritional stresses

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Abstract:

This work was designed to assess regulation of the atf1+ gene in the fission yeast Schizosaccharomyces pombe under nitrosative and nutritional stresses, using the atf1+-lacZ fusion gene and RT-PCR. Nitric oxide (NO)-generating sodium nitroprusside (SNP; 10 µmol/L) and nitrogen depletion significantly enhanced synthesis of -galactosidase from the atf1+-lacZ fusion gene in S. pombe Pap1-positive KP1 cells, but not in S. pombe Pap1-negative TP108-3C cells. SNP (10 µmol/L) and nitrogen depletion also caused a significant increase in atf1+ mRNA levels in Pap1-positive cells, but not in Pap1-negative cells. Depletion of glucose marginally increased synthesis of -galactosidase from the fusion gene in S. pombe Pap1-positive cells. Taken together, the S. pombe atf1+ gene is upregulated by nitrosative and nutritional stresses on a transcriptional level, possibly via the mediation of Pap1.

Ce travail a été conçu pour évaluer la régulation du gène atf1+ chez la levure de fission Schizosaccharomyces pombe soumise à des stress azoté et nutritionnel, à l’aide d’un gène de fusion atf1+-lacZ et par RT-PCR. Le nitroprussiate de sodium générant de l’oxyde nitrique (SNP; 10 µmol/L) et la carence en azote augmentaient significativement la synthèse de ß-galactosidase par le gène de fusion atf1+-lacZ chez S. pombe KP1 positives à Pap1, mais pas chez S. pombe TP108-3 négatives à Pap1. Le SNP (10 µmol/L) et la carence en azote causaient aussi une augmentation significative des niveaux d’ARNm de atf1+ chez les cellules positives à Pap1 contrairement aux cellules négatives à Pap1. La carence en glucose augmentait de façon marginale la synthèse de ß-galactosidase par le gène de fusion chez S. pombe positives à Pap1. En somme, le gène atf1+ de S. pombe est positivement régulé par les stress azoté et nutritionnel au niveau transcriptionel, possiblement par l’intermédiaire de Pap1.

Document Type: Research Article

Publication date: 2009-11-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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