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Role of YiaX2 in l-ascorbate transport in Klebsiella pneumoniae 13882

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The yiaK-S operon is required for aerobic growth on l-ascorbate in several Enterobacteriaceae. Here we present evidence that the yiaX2 gene belonging to the yiaK-S operon of Klebsiella pneumoniae 13882, which encodes a protein similar to the putative transporters classified as the major facilitator superfamily, is involved in the uptake of l-ascorbate. Concentration kinetic analysis yielded an apparent Km of YiaX2 for l-ascorbate of 161.38 ±8.28 µmol·L-1 and a Vmax of 3.81 ± 0.60 nmol·mg-1·min-1. This carrier uses the energy from electrochemical gradients, since it was inhibited by carbonyl cyanide m-chlorophenylhydrazone, a hydrophobic proton conductor that dissipates proton motive force.

L’opéron yiaK-S est requis à la croissance aérobie sur le l-ascorbate de plusieurs bactériacées entériques. Nous présentons ici des preuves qui montrent que le gène yiaX2 appartenant à l’opéron yiaK-S de la souche Klebsiella pneumoniae 13882, qui code une protéine similaire aux transporteurs présumés classés dans la superfamille des facilitateurs majeurs, est impliqué dans la captation du l-ascorbate. Les analyses cinétiques ont donné un Km apparent de YiaX2 pour le l-ascorbate de 161,38 ± 8,28 µmol·L-1 et un Vmax de 3,81 ± 0,60 nmol·mg-1·min-1. Ce transporteur utilise l’énergie des gradients électrochimiques, car il est inhibé par le cyanure de carbonyle m-chlorophénylhydrazone, un conducteur de proton hydrophobe qui dissipe la force protomotrice.

Document Type: Research Article

Publication date: November 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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