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Rapid method for extracting the antibiotic mutacin 1140 from complex fermentation medium yeast extract

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Abstract:

The peptide antibiotic mutacin 1140 belongs to the group of antibiotics called lantibiotics. They are ribosomally synthesized and undergo extensive enzymatic modifications before being excreted into the culture medium. By using reverse-phase - high-performance liquid chromatography (RP-HPLC) and a semiquantitative bacteriocin bioassay to track mutacin 1140, an efficient ammonium sulfate (AS) precipitation method has been developed for removing mutacin 1140 from a complex medium containing 5% yeast extract. This method minimizes the amounts of fermentation by-products and media components that make downstream purification processes more difficult and economically infeasible. The method may be adaptable for the initial purification step of other lantibiotics. A threefold decrease in the precipitation of the medium components found in yeast extract, at pH 8.0 vs. pH 2.0, may have broad utility for the isolation of secondary metabolites produced in this complex medium. The average yield of mutacin 1140 from the fermentation medium was determined as 66%.

La mutacine 1140, un peptide antibiotique, appartient au groupe d’antibiotiques appelés lantibiotiques. Ils sont synthétisés dans le ribosome et subissent de nombreuses modifications enzymatiques avant d’être excrétés dans le milieu de culture. À l’aide du HPLC en phase inversée et d’un test de détection de bactériocine semi-quantitatif pour détecter la mutacine 1140, une méthode de précipitation au sulfate d’ammonium efficace a été développé pour enlever la mutacine 1140 d’un milieu complexe contenant 5 % d’extrait de levure. Cette méthode minimise la quantité de sous-produits de fermentation et de composantes du milieu qui rendent la purification subséquente plus difficile et économiquement infaisable. La méthode peut être adaptée à l’étape de purification initiale d’autres lantibiotiques. Une diminution de trois fois de la précipitation des composantes du milieu trouvées dans l’extrait de levure à pH 8.0 versus pH 2.0 peut avoir une vaste utilité pour isoler des métabolites secondaires produits dans ce milieu complexe. Le rendement de récupération de mutacine 1140 du milieu de fermentation est de 66 %.

Document Type: Research Article

Publication date: 2009-11-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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