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The role of rpoS on the survival of a p-nitrophenol degrading Pseudomonas putida strain in planktonic and biofilm phases

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Abstract:

The survival of and interactions between a Pseudomonas putida strain labelled with a red fluorescent protein gene (WT-rfp) and its green fluorescent protein gene-labelled rpoS- mutant (KO-gfp) were examined. The generation times of the planktonic WT-rfp and KO-gfp in trypticase soy broth were not significantly different (i.e., p > 0.05) from each other at 30 °C. However, the biovolume of the KO-gfp biofilm was about 7 times larger than its WT-rfp counterpart after 48 h of growth. Furthermore, the presence of WT-rfp suppressed the biofilm development of KO-gfp significantly in co-culture biofilms. In planktonic conditions, the pre-carbon-starved WT-rfp achieved a 3-fold greater survival than the pre-carbon-starved KO-gfp in 0.85% saline after a 13-day incubation. In a 1:1 ratio co-culture, the pre-carbon-starved WT-rfp outcompeted the pre-carbon-starved KO-gfp by 20-fold. However, the survival of WT-rfp and KO-gfp were not significantly different from each other in biofilm conditions. Additionally, 11.4% and 61.2% of the WT-rfp and KO-gfp biofilms, respectively, remained intact after washing in 0.2% SDS for 60 min. In conclusion, the rpoS had a significant impact on survival and competitiveness of planktonic P. putida, and on biofilm development, being implicated in competitive suppression of biofilm development in co-culture biofilms and decreased biofilm cohesiveness.

La survie et les interactions entre une souche de Pseudomonas putida marquée par un gène codant la protéine fluorescente rouge (WT-rfp) et un mutant rpoS - marqué à la protéine fluorescente verte (KO-gfp) ont été examinées. Les temps de génération de WT-rfp et de KO-gfp planctoniques dans le milieu trypticase de soja n’étaient pas significativement différents (i.e. p > 0.05) à 30 °C. Cependant, le volume biologique de biofilm formé par la souche KO-gfp était environ 7 fois plus grand que celui de sa contrepartie formée par la souche WT-rfp après 48 h de croissance. De plus, la présence de WT-rfp inhibait le développement du biofilm de KO-gfp de façon significative en co-culture. En conditions planctoniques, la souche WT-rfp carencée en carbone montrait un taux de survie 3 fois plus grand que la souche KO-gfp carencée en carbone en milieu salin 0.85 % après 13 jours d’incubation. Dans un ratio de co-culture 1 : 1, WT-rfp carencée en carbone l’emportait sur KO-gfp carencée en carbone d’un facteur 20. Cependant, les taux de survie de WT-rfp et de KO-gfp n’étaient pas significativement différents l’un de l’autre dans des conditions de formation de biofilms. De plus, 11.4 % de WT-rfp et 61.2 % de KO-gfp en biofilms demeuraient intacts après un lavage au SDS 0.2 % pendant 60 min. En conclusion, le gène rpoS avait un impact significatif sur la survie et la compétitivité de la forme planctonique de P. putida ainsi que dans le développement des biofilms, étant impliqué dans la compétition lors du développement des biofilms en co-culture et dans la diminution de la cohésion des biofilms.

Document Type: Research Article

Publication date: 2009-10-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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