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Detection of Yersinia pestis over time in seeded bottled water samples by cultivation on heart infusion agar

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Abstract:

The viable persistence of Yersinia pestis seeded in bottled spring water was evaluated by performing 2 studies that involved inoculating a total of 21 different test strains into individual 500 mL reservoirs. Approximately 2 × 104 CFU/mL of Y. pestis was inoculated into each reservoir and held for sampling at 26 °C ± 1 °C. In study No. 2, 9 strains (Harbin, Nepal, UNH 1A, UNH 1B, ZE94, CO92, PB6, PB6 DP, and Pexu) could no longer be recovered using a plate count assay between 79 and 138 days post-seeding; other strains (K25 lcr, O19 Ca-6, and K25 pst) could no longer be recovered between 112 and 160 days post seeding. The data generated in this study demonstrate that certain strains of Y. pestis can remain viable in bottled water for extended periods of time.

La persistance de Yersinia pestis viables, ensemencées dans de l’eau de source embouteillée, a été évaluée en réalisant deux études qui impliquaient l’inoculation d’un total de vingt-et-une souches tests différentes dans des contenants de 500 mL. Approximativement 2 × 104 UFC/mL de Y. pestis ont été inoculées dans chaque contenant et gardées pour échantillonnage à 26 ± 1°C. Dans la deuxième étude, neuf souches (Harbin, Népal, UNH 1A, UNH 1B, ZE94, CO92, PB6, PB6 DP, et Pexu) n’étaient plus détectables par un essai de décompte sur plaques, de 79 à 138 jours après l’ensemencement alors que d’autres souches (K25 lcr, O19 Ca-6 et K25 pst) n’étaient plus détectables de 112 à 160 jours après l’ensemencement. Les données engendrées dans cette étude démontrent que certaines souches de Y. pestis demeurent viables dans l’eau embouteillée pendant de longues périodes de temps.

Document Type: Research Article

Publication date: 2009-09-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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