A refrigeration temperature of 4 °C does not prevent static growth of Yersinia pestis in heart infusion broth

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Abstract:

Multiple barriers such as inspections, testing, and proper storage conditions are used to minimize the risk of contaminated food. Knowledge of which barriers, such as refrigeration, are effective in preventing pathogen growth and persistence, can help direct the focus of efforts during food sampling. In this study, the doubling times were evaluated for 10 strains of Yersinia pestis of different genetic background cultured in heart infusion broth (HIB) kept at 4 °C ± 1 °C under static conditions. Nine out of the 10 strains were able to grow at 4 °C ± 1 °C. Apparent doubling times for 7 of the strains ranged from 41 to 50 h. Strain Harbin and strain D1 had apparent doubling times of 65 and 35 h, respectively, and strain O19 Ca-6 did not grow at all. Analysis of variance showed that the averaged growth data (colony forming units per mL) between strains that grew were not significantly different. The data presented here demonstrate that refrigeration alone is not an effective barrier to prevent static growth of Y. pestis in HIB. These findings provide the preliminary impetus to investigate Y. pestis growth in a variety of food matrices that may provide a similar environment as HIB.

De multiples mesures, notamment des inspections, des tests et des conditions d’entreposage adéquates, sont utilisées pour minimiser les risques de contamination des aliments. L’identification de mesures efficaces pour prévenir la croissance et la persistance des pathogènes comme la réfrigération, peut aider à concentrer les efforts lors de prélèvements alimentaires. Dans cette étude, le temps de doublement de 10 souches de Yersinia pestis possédant des backgrounds génétiques différents, cultivées sur une gélose à l’infusion de cœur (HIB, Heart Infusion Broth) et gardées à 4 ± 1°C en conditions statiques a été évalué. Neuf des dix souches pouvaient pousser à 4 ± 1°C. Le temps de doublement apparent de sept souches s’échelonnait de 41 à 50h. La souche Harbin et la souche D1 avaient un temps de doublement apparent de 65 et 35h respectivement, et la souche O19 Ca-6 ne poussait pas du tout. Une analyse de variance (ANOVA) a montré que les données de croissance moyenne des souches qui poussaient, en termes de UFC/ml, n’étaient pas significativement différentes les unes des autres. Les données présentées ici démontrent que la réfrigération seule n’est pas une barrière efficace pour prévenir la croissance statique de Y. pestis dans le HIB. Ces résultats incitent à examiner la croissance de Y. pestis dans une variété de matrices alimentaires qui pourraient fournir un environnement similaire au HIB.

Document Type: Research Article

Publication date: September 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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