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Phylogenetic analysis of methanotrophic communities in cover soils of a landfill in Ontario

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Abstract:

We examined the methanotrophs in the Trail Road Landfill soils, Ottawa, Ontario, through cultivation-independent molecular assay and the culturing approach. Denaturing gradient gel electrophoresis (DGGE) analysis of amplified methanotroph-specific 16S rDNA gene fragments revealed a more diverse type I (RuMP pathway) methanotrophic community than type II (serine pathway) in 17 soil samples taken along a 50 m transect. The type II methanotrophic community was less diverse, with the dominance of Methylocystis in almost all samples, and clustering with high similarity (85%-88%). Also, the results showed that the C/N ratio of soil organic matter could significantly affect the methanotrophic community structures. The DGGE results were supported by sequence analysis of cloned pmoA. Members of the genera Methylobacter (type I), Methylocaldum (type X), and Methylocystis (type II) appeared to be the dominant methanotrophs. From methanotrophic enrichments, we isolated type I Methylobacter sp., and 3 type II Methylocystis spp.,which appeared to be one of the dominant bacteria species in the soil sample from which isolates were obtained.

Nous avons examiné les méthanotrophes retrouvés dans des sols provenant de la décharge Trail Road à Ottawa, Ontario, à l’aide de techniques moléculaires ne nécessitant pas la culture de micro-organismes, et aussi à l’aide de cultures. La communauté méthanotrophe de type II était moins diversifiée, et la dominance de Methylocystis a été observée dans presque tout les échantillons, et était regroupée dans un amas avec une similarité élevée (85 %-88 %). Aussi, les résultats ont montré que le ratio C/N de la matière organique du sol pouvait affecter de manière significative la structure de la communauté méthanotrophe. Les résultats obtenus à l’aide du DGGE sont supportés par l’analyse de séquence pmoA clonées. Les membres des genres Methylobacter (type I), Methylocaldum (type X) et Methylocystis (type II) sont apparus comme étant les méthanotrophes dominants. À partir d’enrichissements méthanotrophes, nous avons isolé une souche de Methylobacter sp. de type I et 3 Methylocystis de type II qui semblait être une des espèces bactériennes dominantes dans l’échantillon de sol à partir duquel des isolats ont été obtenus.

Document Type: Research Article

Publication date: September 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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