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The role of Acidithiobacillus ferrooxidans in alleviating the inhibitory effect of thiosulfate on the growth of acidophilic Acidiphilium species isolated from acid mine drainage samples from Garubathan, India

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Abstract:

Several acidophilic chemolithoautotrophic and heterotrophic strains were isolated from acid mine drainage samples from Garubathan, West Bengal, India. The strains, chemolithoautotrophic DK6.1 and heterotrophic DKAP1.1, used in this study were assigned to the species Acidithiobacillus ferrooxidans and Acidiphilium cryptum, respectively. Unamended filtered and subsequently autoclaved elemental sulfur spent medium of A. ferrooxidans was used as the medium to study heterotrophic growth of A. cryptum DKAP1.1. While characterizing the heterotrophic strain, an inhibitory effect of thiosulfate on A. cryptum DKAP1.1 was identified. The lethality of thiosulfate broth was directly related to the concentration of thiosulfate in the medium. Nonviability of A. cryptum DKAP1.1 in the presence of thiosulfate was alleviated by A. ferrooxidans DK6.1 in co-culture. Microbiological data on a positive growth effect for A. ferrooxidans DK6.1 caused by co-culturing in solid media in the presence of A. cryptum DKAP1.1 is also presented.

Plusieurs souches acidophiles chimiolithoautotrophes et hétérotrophes ont été isolées de neuf échantillons d’exhaure acide à Garubathan, Bengale de l’Ouest, Inde. Les souches chimiolithoautrophe DK6.1 et hétérotrophe DKAP1.1 utilisées dans cette étude ont été respectivement attribuées aux espèces Acidithiobacillus ferrooxidans et Acidiphilium cryptum. Le milieu de A. ferrooxidans épuisé en soufre élémentaire, non amendé et subséquemment autoclavé, a été utilisé pour étudier la croissance hétérotrophe de A. cryptum DKAP1.1. En caractérisant cette souche hétérotrophe, un effet inhibiteur du thiosulfate sur A. cryptim DKAP1.1 a été observé. La létalité du thiosulfate a été directement reliée à la concentration de thiosulfate du milieu. La non-viabilité de A. cryptum DKAP1.1 en présence de thiosulfate a été réduite par A. ferrooxidans DK6.1 en co-culture. Des données microbiologiques de l’effet positif sur la croissance de A. ferrooxidans DK6.1 produit par la co-culture en milieu solide en présence de A. cryptum DKAP1.1 ont aussi été présentées.

Document Type: Research Article

Publication date: September 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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