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Enhancement of surfactin production of Bacillus subtilis fmbR by replacement of the native promoter with the Pspac promoter

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Abstract:

Bacillus subtilis fmbR-1 was obtained from wild-type B. subtilis fmbR by replacement of the native promoter of the surfactin operon with the inducible promoter Pspac. The recombinant B. subtilis fmbR-1 produced more antibacterial substances than the wild-type strain. The overproducing phenotype was related to the enhancement of antagonistic activities against Bacillus cereus. HPLC peaks of surfactin for recombinant fmbR-1 showed patterns of lipopeptides similar to those of the wild-type strain, and surfactin production of the recombinant fmbR-1 was up to about fivefold greater than that of the wild-type strain without induction by isopropyl -d-1-thiogalactopyranoside. However, the production of surfactin increased to about 10-fold more than that of the wild-type strain when it was induced by isopropyl -d-1-thiogalactopyranoside.

Une souche Bacillus subtilis fmbR-1 a été obtenue à partir de B. subtilis fmbR sauvage en remplaçant le promoteur natif de l’opéron de la surfactine par le promoteur inductible Pspac. La souche recombinante B. subtilis fmbR-1 produisait davantage de substances anti-bactériennes que le type sauvage. Le phénotype de surproduction était en lien avec l’augmentation des activités antagonistes envers Bacillus cereus. Les pics de surfactine du recombinant fmbR-1 détectés en HPLC montraient des patrons de lipopeptides similaires à ceux du type sauvage et la production de surfactine par le recombinant fmbR-1 était presque cinq fois plus élevée que celle du type sauvage sans induction par le -d-1-thiogalactopyranoside d’isopropyle. Cependant, la production de surfactine était environ 10 fois plus élevée que celle de la souche sauvage lorsqu’elle était induite par le -d-1-thiogalactopyranoside d’isopropyle.

Document Type: Research Article

Publication date: August 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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