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Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis

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Abstract:

A prospective study was designed to evaluate the clinical usefulness of spoligotyping applied directly to sputum samples. Patients suspected of having tuberculosis were recruited at the Hospital Sanatorio Partenon in Porto Alegre, Brazil. Of the 197 samples included in the analysis, 175 (88.8%) yielded a spoligotyping result that fully matched that obtained from culture. Low bacillary samples presented lower accuracy (50%). From 135 Mycobacterium tuberculosis spoligopatterns, we identified 44 different spoligotypes, of which 21 were shared patterns and 23 were unique. T1 was the most frequent subfamily. The genotyping strategy proposed here presents a short turnaround time and could be helpful in providing rapid information on strain identities in a clinical setting.

Une étude prospective a été conçue pour évaluer l’utilité clinique du spoligotypage (pour spacer-oligonucleotide-typing) appliqué directement à des échantillons d’expectoration. Des patients soupçonnés de tuberculose ont été recrutés à l’Hospital Sanatorio Partenon à Porto Allegre, Brésil. Des 197 échantillons inclus dans l’analyse, 175 (88,8 %) ont généré un résultat de spoligotypage qui concordait parfaitement avec les résultats obtenus en culture. Les échantillons faiblement bacillaires présentaient une fiabilité plus faible (50 %). À partir des 135 patrons de spoligotypage de Mycobacterium tuberculasis, nous avons identifié 44 spoligotypes différents, parmi lesquels 21 étaient partagés et 23 étaient uniques. T1 constituait la sous-famille la plus fréquente. La stratégie de génotypage proposée ici offre un temps de réalisation court et pourrait être utile en générant rapidement de l’information sur l’identité des souches dans un cadre clinique.

Document Type: Research Article

Publication date: 2009-07-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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