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Involvement of RecG in H2O2-induced damage repair in Deinococcus radiodurans

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Abstract:

Deinococcus radiodurans (ex Raj et al. 1960) Brooks and Murray 1981 is well known for its efficient repair of various types of physically or chemically induced DNA damage caused by ionizing and ultraviolet radiation or H2O2. RecG codes for a helicase that is known to be involved in repairing oxidative damage in other bacterium. In this work, we constructed a DRrecG deletion mutant and investigated its possible role in H2O2-induced damage. The results showed that the deletion of DRrecG resulted in an obvious growth defect and great decrease of radioresistance of D. radiodurans to gamma radiation and H2O2. We also defined the transcriptional profiles of the recG mutant and wild-type strain with and without treatment with H2O2. These results suggested that DRrecG is important for DNA repair during oxidative damage.

Deinococcus radiodurans (ex Raj et al. 1960) Brooks and Murray 1981 est bien connue pour sa réparation efficace de divers types de dommages physiquement ou chimiquement induits à l’ADN par les radiations ionisantes et ultraviolettes ou par l’H2O2. RecG code une hélicase impliquée dans la réparation du dommage oxydatif chez une autre bactérie. Dans ce travail, nous avons construit un mutant de délétion de DRrecG et nous avons examiné son rôle potentiel dans les dommages induits par le H2O2. Les résultats on montré que la délétion de DRrecG résultait en un défaut de croissance évident et en une diminution de la radiorésistance de D. radiodurans aux radiations gamma et au H2O2. Nous avons aussi défini le profil transcriptionnel du mutant recG et de la souche sauvage en présence et en absence de traitement au H2O2. Ces résultats suggèrent que DRrecG est important à la réparation d’ADN lors du dommage oxydatif.

Document Type: Research Article

Publication date: July 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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