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Amplified ribosomal DNA restriction analysis of free-living bacteria present in the headbox of a Canadian paper machine

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Abstract:

The headbox water is the main source of bacterial contamination of paper machines. Identification of these bacterial contaminants could be an asset in developing specific control methods. An amplified ribosomal DNA restriction analysis (ARDRA) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a Canadian mill in February and July 2006. Eight bacterial genera were identified as the main colonizers present in the headbox water. The genus Meiothermus appeared to be the dominant bacterial group in the Canadian paper machine. Some variation was observed between the February and July clone libraries. Bacterial genera such as Chelatococcus and Hydrogenophilus were only detected in February or in July, respectively. Furthermore, the proportion of Tepidimonas clones in the libraries was higher in July than in February. The metabolic profile of the February and July communities, determined using Biolog EcoPlates, also suggested that temporal variation occurred within the bacterial populations that colonized the headbox of the paper machine.

La principale source de contamination bactérienne des machines à papier est l’eau de la caisse d’arrivée. L’identification des contaminants bactériens pourrait constituer un atout pour développer des méthodes de contrôle spécifiques. Une analyse des fragments de restriction de l’ADN ribosomal amplifié (ARDRA, pour amplified ribosomal DNA restriction fragment analysis) a été réalisée pour caractériser les communautés bactériennes associées à l’eau de la caisse d’arrivée d’une machine à papier dans un moulin au Canada, en février et en juillet 2006. Huit genres bactériens ont été identifiés comme colonisateurs principaux de l’eau de la caisse d’arrivée. Le genre Meiothermus semblait constituer le groupe bactérien dominant de la machine à papier canadienne. Quelques variations ont été observées entre les banques de clones constituées en février et en juillet. Les genres bactériens tels Chelatococcus et Hydrogenophilus n’étaient détectés respectivement qu’en février et en juillet. En outre, la proportion de clones de Tepidimonas dans les banques était plus élevée en juillet qu’en février. Le profil métabolique des communautés de février et de juillet, déterminé sur des microplaques Biolog EcoPlates suggérait aussi qu’il existait une variation temporelle au sein des populations bactériennes qui colonisaient la caisse d’arrivée de le machine à papier.

Document Type: Research Article

Publication date: July 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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