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Clonal relationships determined by multilocus sequence typing among enteropathogenic Escherichia coli isolated in Brazil

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Abstract:

Enteropathogenic Escherichia coli (EPEC) infections are a leading cause of infantile diarrhea in developing nations. Multilocus sequence typing (MLST) characterizes bacterial strains based on the sequences of internal fragments in housekeeping genes. Little is known about strains of EPEC analyzed by MLST from Brazil. In this study, a diverse collection of 29 EPEC strains isolated from patients with diarrhea, admitted to the University Hospital of Ribeirao Preto, was characterized by MLST. Strain analysis demonstrated 22 different sequence types (STs), of which almost half (48%) were new, indicating a high genotype diversity. The 22 STs were divided by eBURST into 12 clonal complexes. It was not possible to correlate typical and atypical EPEC with other strains in the MLST database. This is the first study that analyzed EPEC strains from South America that are included in the E. coli MLST database. Nine (31%) out of 29 strains are part of the CC10 clonal complex, the major clonal complex in the database, which comprises 174 strains and 86 different STs, suggesting that these strains might be the most important intestinal pathogenic E. coli worldwide. Genetic relationships between typical and atypical EPEC, enterohemorrhagic E. coli, and enteroaggregative E. coli strains were not established by MLST.

Les infections à Escherichia coli entéropathogène (EPEC) sont les principales causes de la diarrhée infantile dans les pays en voie de développement. Le typage de séquences multilocus (multilocus sequence typing ou MLST) permet de caractériser les souches bactériennes en fonction des séquences de fragments internes de gènes domestiques. On connaît peu de choses des souches EPEC analysées par MLST au Brésil. Dans cette étude, une collection variée de 29 souches d’EPEC isolées de patients souffrant de diarrhée, admis à l’hôpital universitaire de Ribeirao Preto, a été caractérisée par MLST. L’analyse des souches a révélé la présence de 22 types différents de séquences, parmi lesquels près de la moitié (48 %) étaient nouveaux, indiquant une haute diversité génotypique. Les 22 types de séquences ont été divisés par eBURST en 12 complexes clonaux. Il n’a pas été possible de corréler les EPEC typiques et atypiques avec les autres souches dans la base de données du MLST. Cette étude est la première à analyser les souches d’EPEC d’Amérique du sud comprises dans le MLST de E. coli. Neuf (31 %) des 29 souches font partie du complexe clonal CC10, qui est le complexe clonal principal de la base de données qui comprend 174 souches et 86 types de séquences différents, ce qui suggère que ces souches peuvent constituer le groupe d’E. coli pathogène intestinal le plus important dans le monde. Les relations génétiques entre les souches EPEC, E. coli entéro-hemorragique et E. coli entéro-agrégant typiques et atypiques n’ont pas été établies par le MLST.

Document Type: Research Article

Publication date: June 1, 2009

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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