Lactobacillus casei: influence on the innate immune response and haemostatic alterations in a liver-injury model

Authors: Haro, Cecilia; Zelaya, Hortensia; Lazarte, Sandra; Alvarez, Susana; Agüero, Graciela

Source: Canadian Journal of Microbiology, Volume 55, Number 6, June 2009 , pp. 648-656(9)

Publisher: NRC Research Press

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Abstract:

Lactobacilllus casei CRL 431 has the ability to modulate the local and systemic immune responses, which are significantly involved in liver injury caused by hepatotoxins. This work was conducted to determine whether L. casei has a preventive effect on the hepatic damage undergone during an acute liver injury (ALI). Methods: ALI was induced by an intraperitoneal injection of d-galactosamine (D-Gal). Different groups of mice received 1× 109 L. casei cells/day/mouse for 2 days before D-Gal injection. Blood and liver samples were obtained 0, 6, 12, and 24 h after D-Gal administration. Results: D-Gal induced increases in serum aminotransferases, reduced the number of blood leukocytes, enhanced neutrophil myeloperoxidase activity, increased dead cells, and altered prothrombin time and plasma fibrinogen levels. The preventive treatment with L. casei for 2 days modulated the innate immune response. This effect was shown by the earlier normalization of white blood cell counts, myeloperoxidase activity and aminotransferases levels. However, the haemostatic parameters were only partially recovered. The favourable effects obtained could be due to the capacity of L. casei to moderate the inflammatory response at the site of the injury with less damage to liver tissue.

Lactobacillus casei CRL 431 a la capacité de moduler les réponses immunes locale et systémique, lesquelles sont significativement impliquées dans le dommage au foie causé par les hépatotoxines. Ce travail a été réalisé pour déterminer si L. casei pouvait prévenir des dommages hépatiques subits lors d’une atteinte hépatique aiguë (AHA). Méthode : l’AHA a été induite par une injection intrapéritonéale de d-galactosamine (D-Gal). Différents groupes d’animaux ont reçu 1 × 109 cellules/jour/souris (L. casei) pendant 2 jours avant l’injection de D-Gal. Des échantillons de sang et de foie ont été prélevés 0, 6, 12 et 24 h après l’administration de D-Gal. Résultats : La D-Gal a induit une augmentation de l’activité des aminotransférases sériques, une réduction du nombre de leucocytes sanguins et une augmentation de l’activité de la myéloperoxydase des neutrophiles. Un traitement préventif à L. casei pendant 2 jours a contribué à moduler la réponse immune innée. Cet effet a été mis en évidence par la normalisation précoce de globules blancs sanguins, de l’activité de la MPO ainsi que des niveaux d’aminotransférases. Cependant, les paramètres hémostatiques n’étaient que partiellement recouvrés. Les effets favorables obtenus pourraient être dus à la capacité de L. casei à moduler la réponse inflammatoire au site atteint, résultant en un dommage moindre du tissu hépatique.

Document Type: Research Article

Publication date: June 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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