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The role of heparan sulfate on adhesion of 47 and 51 kDa outer membrane proteins of Helicobacter pylori to gastric cancer cells

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Abstract:

Helicobacter pylori is a common gastrointestinal pathogenic bacterium in humans and the usual preference for the stomach’s outer membrane proteins (OMPs) are antigens involved in the adhesion process. Through SDS-PAGE and blotting analyses, using horseradish peroxidase-labeled heparan sulfate (HRP-HS) as a probe, we identified H. pylori OMPs with affinity for heparan sulfate (OMP-HS). Biotin-streptavidin bacterial-adhesion assay was used to evaluate participation of OMP-HS in the adhesion of H. pylori to semi-confluent HeLa S3 and Kato III cell monolayers. The results provide evidence that induction of antibodies against 2 OMP-HSs (HSBP-47 and HSBP-51) could reduce binding of H. pylori to both cell lines and induce detachment of cell-bound bacteria from infected cultured cells.

Helicobacter pylori est une bactérie pathogène communément retrouvée dans le tractus gastro-intestinal chez l’humain et les protéines membranaires externes (OMP, acronyme de outer membrane proteins) des bactéries retrouvées habituellement dans l’estomac consistent en antigènes impliqués dans le processus d’adhérence. Des analyses réalisées en SDS-PAGE et par immuno-buvardage à l’aide d’une héparane sulfate marquée à la peroxydase de raifort nous ont permis d’identifier des OMP de H. pylorii possédant une affinité pour l’héparane sulfate (OMP-HS). Un essai d’adhérence bactérienne à l’avidine-biotine a été utilisé pour évaluer la participation de ces OMP-HS dans l’adhérence de H. pylori à des monocouches de cellules HeLa S3 et Kato III semi-confluentes. Les résultats mettent en évidence que l’induction d’anticorps dirigés contre 2 OMP-HS (HSBP-47 et HSBP-51) pourrait réduire la liaison de H. pylori aux deux lignées cellulaires et induire le détachement des bactéries adhérentes des cellules en culture infectées.

Document Type: Research Article

Publication date: April 1, 2009

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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