Skip to main content

Repetitive element (REP)-polymerase chain reaction (PCR) analysis of Escherichia coli isolates from recreational waters of southeastern Lake Huron

Buy Article:

$50.00 plus tax (Refund Policy)

Abstract:

Repetitive element-polymerase chain reaction (REP-PCR) DNA fingerprinting and library-based microbial source tracking (MST) methods were utilized to investigate the potential sources of Escherichia coli pollution in recreational waters of southeastern Lake Huron. In addition to traditional sources such as humans, agriculture, and wildlife, environmentally persistent E. coli isolates were included in the identification library as a separate library unit consisting of the E. coli strains isolated from interstitial water on the beach itself. Our results demonstrated that the dominant source of E. coli pollution of the lake was agriculture, followed by environmentally adapted E. coli strains, wildlife, and then humans. A similar ratio of contributing sources was observed in all samples collected from various locations including the river discharging to the beach in both 2005 and 2006. The high similarity between the compositions of E. coli communities collected simultaneously in the river and in the lake suggests that tributaries were the major overall sources of E. coli to the lake. Our findings also suggest that environmentally adapted strains (EAS) of E. coli should be included as one of the potential sources in future microbial source tracking efforts.

L’empreinte ADN par REP-PCR (Repetitive element-polymerase chain reaction) et l’identification des sources de contamination par MST (Microbial source tracking) à partir de banques ont été utilisées pour investiguer les sources potentielles de pollution par E. coli dans les eaux de baignade sud-est du Lac Huron (Canada). En plus des sources traditionnelles que sont l’humain, l’agriculture et la faune, des isolats persistants d’E. coli consistant en souches d’E. coli isolées des eaux interstitielles de la plage elle-même ont été inclus dans la banque d’identification comme unités indépendantes. Nos résultats ont démontré que la source dominante de pollution par E. coli du lac était l’agriculture, suivie par les souches d’E. coli adaptées à l’environnement, la faune et finalement, l’humain. Un ratio similaire de sources contribuant à la pollution a été observé dans tous les échantillons recueillis à différents endroits, y compris à la décharge de la rivière en 2005 et 2006. Le haut niveau de similarité dans la composition des communautés d’E. coli recueillies simultanément dans la rivière et le lac suggère que les affluents sont les sources générales majeures d’E. coli du lac. Nos résultats suggèrent aussi que les souches d’E. coli adaptées à l’environnement devraient être incluses parmi les sources potentielles de contamination microbienne dans les protocoles de MST futurs.

Document Type: Research Article

Publication date: March 1, 2009

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
nrc/cjm/2009/00000055/00000003/art00007
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more