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Identification and potential enzyme capacity of flavobacteria isolated from the rhizosphere of barley (Hordeum vulgare L.)

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Abstract:

The diversity of 99 flavobacterium-like isolates from a barley rhizosphere is described. They were identified on 1/10 strength tryptic soy agar by their yellowish colour and a flexirubin reaction after exposure to 10% KOH.16S rDNA partial sequencing identified the majority (70%) of isolates as Flavobacterium. Twelve percent of the isolates belonged to other genera in the phylum Bacteroidetes. Finally 17% of the isolates did not belong to the phylum Bacteroidetes. Most of the Flavobacterium isolates were affiliated to various aquatic, validly named species and likely represent a bulk of undescribed soil Flavobacterium species found in especially high numbers in the rhizosphere of young plant roots. Most Flavobacterium isolates showed gliding motility on CY agar and VY/2 agar, whereas none of the other isolates shared this feature. A high percentage of Flavobacterium strains produced enzymes involved in polysaccharide and protein digestion as well as extracellular phosphatases, compared with strains related to other genera in the phylum Bacteroidetes.

La diversité de 99 isolats de type flavobacterium présents dans la rhizosphère de l’orge est décrite. Ils ont été identifiés sur une gélose tryptique-soja 1/10 par leur couleur jaunâtre et par une réaction de la flexirubine après une exposition au KOH 10%. Le séquençage partiel de l’ADNr 16S a permis d’attribuer la majorité des isolats (70 %) à Flavobacterium. Douze pourcent des isolats appartenaient à d’autres genres du phylum des Bacteroidetes. Finalement, 17 % des isolats n’appartenaient pas au phylum des Bacteroidetes. La plupart des isolats de Flavobacterium étaient affiliés à diverses espèces aquatiques dûment identifiées et représentent probablement l’essentiel des espèces non décrites de Flavobacterium du sol particulièrement trouvées en grand nombre dans la rhizosphère des racines des jeunes plants. La plupart des isolats de Flavobacterium étaient motiles, glissant à la surface des géloses CY et VY/2 alors qu’aucun des autres isolats ne partageait cette caractéristique. Un fort pourcentage de souches de Flavobacterium produisait des enzymes impliquées dans la digestion des protéines et des polysaccharides ainsi que des phosphatases extracellulaires, comparativement aux souches reliées à d’autres genres du phylum des Bacteroidocetes.

Document Type: Research Article

Publication date: March 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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