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Detection of Bacillus sphaericus mosquitocidal toxin genes by multiplex colony PCR

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A multiplex colony PCR assay was developed for the detection of 5 genes encoding Bacillus sphaericus mosquito larvicidal toxins, namely binA, binB, mtx1, mtx2, and mtx3. Primers designed for these 5 genes yielded specific PCR amplicons of the expected size from type cultures of B. sphaericus. This method of detecting multiple toxin genes by colony PCR in a single tube reaction is a simple, rapid, and economical technique for identification of highly toxic environmental B. sphaericus isolates.

Une PCR multiplex de colonies a été développée afin de détecter cinq gènes codant les toxines binA, binB, mtx1, mtx2 et mtx3 de Bacillus sphaericus, larvicides chez le moustique. Les amorces conçues pour ces cinq gènes ont généré des amplicons en PCR spécifiques, de la taille attendue, chez les cultures de B. sphaericus. Cette méthode de détection de plusieurs gènes codant des toxines par des PCR de colonies dans une seule éprouvette est une technique simple, efficace et économique pour identifier des isolats de B. sphaericus de l’environnement hautement toxiques.

Document Type: Research Article

Publication date: February 1, 2009

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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