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Whole-cell protein and ITS rDNA profiles as diagnostic tools to discriminate Fusarium avenaceum intraspecific variability and associated virulence

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Abstract:

A total of 91 isolates of Fusarium avenaceum were regrouped into 15 phenotypes and 10 vegetative compatibility groups showing specific one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (1-D SDS-PAGE) protein profiles and less-specific internal transcribed spacer rDNA profiles. Each isolate possessed reproducible signature protein bands. Indeed, the unweighted pair group method with arithmetic averages clustering revealed that the protein profile of each group of isolates correlated with fungus virulence. The use of SDS-PAGE offers a simple and sensitive technique for routine differentiation between pathogenic and nonpathogenic isolates within unknown F. avenaceum populations. The discovery has significant implications for risk assessment of cereal yield to ensure food and feed safety. This low-cost approach has the potential to be optimized and extended to a broad spectrum of Fusarium head blight pathogens.

Un total de 91 isolats de Fusarium avenaceum ont été regroupés en 15 phénotypes et en 10 groupes de compatibilité végétative, présentant en électrophorèse sur gel de polyacrylamine-sodium docécyl sulfate à un dimension (SDS-PAGE 1-D) des profils protéiques spécifiques mais des profils d’ADNr de l’espaceur interne transcrit (ITS) moins spécifiques. Chaque isolat possédait une signature reproductible quant aux bandes de protéines. En effet, l’algorithme de regroupement « unweighted pair group method with arithmetic averages » a révélé que le profil protéique de chaque groupe d’isolats était corrélé avec la virulence du champignon. L’utilisation de SDS-PAGE offre une technique simple et sensible pour différencier de routine les isolats pathogènes des isolats non pathogènes au sein de populations inconnues de F. avenaceum. Cette découverte a des implications significatives pour l’évaluation des risques dans un contexte de sécurité alimentaire des récoltes de céréales. Cette approche à bas coût peut être optimisée et étendue à un large spectre de pathogènes associés à la fusariose des panicules.

Document Type: Research Article

Publication date: February 1, 2009

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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