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Spatial distribution of bacterial communities in sediment of a eutrophic lake revealed by denaturing gradient gel electrophoresis and multivariate analysis

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Abstract:

Bacterial community structure and the effects of several environmental factors on the microbial community distribution were investigated in the sediment of the eutrophic Lake Xuanwu. Profiles of bacterial communities were generated using denaturing gradient gel electrophoresis (DGGE), and the results were interpreted with multivariate statistical analysis. Five major variables in sediment were examined in a principal component analysis, which indicates notable differences of physicochemical parameters among different sites of the lake. To assess changes in the genetic diversity of bacterial communities of different sampling sites, DGGE band patterns were analyzed by multidimensional scaling analysis, which indicated that sampling sites having similar environmental characteristics also have the similar microbial communities. Canonical correspondence analysis demonstrated that pH and redox potential had significant effects on the bacterial community composition in the sediments. Analysis of DNA sequences revealed that the dominant bacterial groups in Lake Xuanwu belonged to Proteobacteria, Actinobacteria, Verrucomicrobia, and Nitrospirae, which are commonly isolated from freshwater ecosystems.

La structure de la communauté bactérienne et les effets de différents facteurs environnementaux sur la distribution de la communauté microbienne ont été étudiés dans les sédiments d’un lac eutrophique, le Lac Xuanwu. Les profils des communautés bactériennes ont été générés par des électrophorèses sur gel en gradient dénaturant (« DGGE ») et les résultats ont été interprétés par des analyses statistiques multivariables. Cinq variables principales du sédiment ont été examinées par des analyses en composantes principales (PCA), qui ont révélé la présence de différences notables dans les paramètres physicochimiques des différents sites du lac. Pour évaluer les changements de la diversité génétique des communautés bactériennes des différents sites d’échantillonnage, les patrons des bandes obtenues en DGGE ont été analysés par échelonnement multidimensionnel (MDS) qui a indiqué que les sites d’échantillonnage ayant des caractéristiques environnementales similaires possédaient aussi des communautés microbiennes similaires. Des analyses canoniques des correspondances (CCA) ont démontré que le pH et le potentiel d’oxydo-réduction avaient des effets significatifs sur la composition des communautés bactériennes des sédiments. Les analyses des séquences d’ADN ont révélé que les groupes bactériens dominants du Lac Xuanwu appartenaient aux Proteobacteria, Actinobacteria, Verrucomicrobia et Nitrospirae, qui sont couramment isolés des écosystèmes d’eau douce.

Document Type: Research Article

Publication date: December 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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