Heat-shock-induced oxidative stress and antioxidant response in Aspergillus niger 26

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Abstract:

To extend the knowledge about the relationship between heat shock and oxidative stress in lower eukaryotes, the filamentous fungus Aspergillus niger 26 was chosen as a model system. Here, the response of A. niger cells to heat shock is reported. The temperature treatment significantly increased the levels of reactive oxygen species, superoxide anions (O2•-), and hydrogen peroxide and the rate of cyanide-resistant respiration as a marker of oxidative stress. Enhanced reactive oxygen species generation coincided with an increase in the content of oxidative damaged protein and in the accumulation of the storage carbohydrates trehalose and glycogen. Thermal survival of the A. niger cells corresponded to a significant increase in the levels of the antioxidant enzymes superoxide dismutase and catalase for all variants. These observations suggest that heat and oxidative stress have a common cellular effect.

Afin d’étendre nos connaissances de la relation qui existe entre le choc thermique et le stress oxydatif chez les eucaryotes inférieurs, le champignon filamenteux Aspergillus niger 26 a été choisi comme modèle. La réponse d’A. niger au choc thermique est décrite ici. Un traitement thermique a augmenté de façon significative les niveaux d’espèces réactives d’oxygène, les anions superoxyde (O2•-) et le peroxyde d’hydrogène, ainsi que le taux de respiration résistante au cyanure utilisé comme marqueur du stress oxydatif. L’augmentation de la production d’espèces réactives d’oxygène coïncidait avec l’augmentation du contenu en protéines endommagées par l’oxydation et l’accumulation de glucides, le tréhalose et le glycogène. La survie à haute température d’A. niger correspondait à une augmentation significative des niveaux des enzymes antioxydantes, la SOD et la CAT, chez tous les variants. Ces observations suggèrent que la chaleur et le stress oxydatif ont certains effets cellulaires en commun.

Document Type: Research Article

Publication date: December 1, 2008

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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