Identification of genes differentially expressed in a strain of the mold Aspergillus nidulans carrying a loss-of-function mutation in the palA gene

Authors: Silva, Emiliana M.; Freitas, Janaína S.; Gras, Diana E.; Squina, Fábio M.; Leal, Juliana; Silveira, Henrique C.S.; Martinez-Rossi, Nilce M.; Rossi, Antonio

Source: Canadian Journal of Microbiology, Volume 54, Number 10, October 2008 , pp. 803-811(9)

Publisher: NRC Research Press

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Abstract:

To identify genes differentially expressed in a strain of the mold Aspergillus nidulans carrying a loss-of-function mutation in palA, a gene in the pH-responsive signal transduction pathway, suppression subtractive hybridization was performed between RNA isolated from the biA1 and biA1 palA1 strains grown under limiting inorganic phosphate at pH 5.0. We have identified several genes upregulated in the biA1 palA1 mutant strain that play important roles in mitotic fidelity, stress responses, enzyme secretion, signal transduction mechanisms, development, genome stability, phosphate sensing, and transcriptional regulation among others. The upregulation of eight of these transcripts was also validated by Northern blot. Moreover, we show that a loss of function mutation in the palA gene drastically reduced the neutral sugar content of the acid phosphatase PacA secreted by the fungus A. nidulans grown at pH 5.0 compared with a control strain.

Afin d’identifier les gènes exprimés de façon différentielle chez une souche de la moisissure Aspergillus nidulans comportant une mutation avec perte de fonction de palA, un gène impliqué dans la transduction de signaux dépendants du pH, une hybridation suppressive soustractive a été réalisée entre l’ARN isolé des souches biA1et biA1 palA1 cultivées sous des conditions de phosphate inorganique limitantes à pH 5,0. Nous avons identifié plusieurs gènes régulés positivement chez la souche mutante biA1 palA1, qui jouent un rôle important notamment dans la fidélité de la mitose, les réponses au stress, la sécrétion d’enzymes, les mécanismes de transduction de signaux, le développement, la stabilité du génome, la perception du phosphate et la régulation de la transcription. La régulation positive de huit de ces transcrits a aussi été validée par buvardage Northern. Nous démontrons de plus qu’une mutation avec perte de fonction du gène palA diminue dramatiquement le contenu en sucre neutre de la phosphatase acide PacA secrétée par le champignon A. nidulans cultivé à pH 5,0 comparativement à la souche contrôle.

Document Type: Research Article

Publication date: October 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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