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Variation of cell surface hydrophobicity and biofilm formation among genotypes of Candida albicans and Candida dubliniensis under antifungal treatment

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Candida infections are frequently associated with formation of biofilms on artificial medical devices. This work studied variation of cell surface hydrophobicity (CSH) and formation of biofilm in relation to Candida albicans and Candida dubliniensis genotypes and an effect of some conventional antifungal agents on both CSH and biofilm. The 50 isolates of C. albicans and C. dubliniensis were classified into genotypes A, B, C, and D, genotype D being exclusively represented by C. dubliniensis. No significant differences between CSH of genotypes A and B and B and C were observed with respect to cultivation temperature 25 or 37 °C. Candida dubliniensis showed increased CSH in comparison with other C. albicans genotypes (p < 0.001) regardless of temperature used. Using XTT reduction assay and dry masses, genotypes B and C showed reduced ability to form biofilm in comparison with genotype A (p < 0.05) and C. dubliniensis (p < 0.001). Fluconazole reduced biofilm in C. albicans genotypes A, B, and C (p < 0.05) but not CSH. The opposite effect was observed in C. dubliniensis. Voriconazole effectively reduced both biofilm formation and CSH in all tested genotypes of C. albicans and C. dubliniensis (p < 0.05).

Les infections à Candida sont fréquemment associées à la formation d’un biofilm sur du matériel médical artificiel. Cette étude s’est penchée sur la variation de l’hydrophobicité de la surface cellulaire (HSC) et sur la formation d’un biofilm en fonction des génotypes de Candida albicans et Candida dubliniensis, ainsi que sur l’effet d’agents antifongiques conventionnels sur l’HSC et la formation de biofilm. Les 50 isolats de C. albicans et C. dubliniensis ont été classifiés en génotypes A, B, C, et D, ce dernier étant exclusivement représenté par C. dubliniensis. Aucunes différences significatives n’ont été observées entre l’HSC des génotypes A et B et des génotypes B et C, en fonction des températures de culture, à 25 ou 37  °C. Une HSC plus élevée a été observée chez C. dubliniensis comparativement aux autres génotypes de C. albicans (p < 0,001), peu importe la température utilisée. Selon les résultats d’un essai de réduction du XTT et selon le poids sec, les génotypes B et C possédaient une capacité réduite de formation de biofilm, comparativement aux génotypes A (p < 0,05) et à C. dubliniensis (p < 0,001). Le fluconazole a réduit la production de biofilm des génotypes A, B et C de C. albicans (p < 0,05), mais pas l’HSC. L’effet contraire a été observé chez C. dubliniensis. Le voriconazole a réduit efficacement tant la formation de biofilm que l’HSC chez tous les génotypes de C. albicans et de C. dubliniensis (p < 0,05).

Document Type: Research Article

Publication date: September 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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