Candida albicans-secreted lipase induces injury and steatosis in immune and parenchymal cells

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Abstract:

Virulence depends on opposing reactions between host and pathogen and is intrinsically linked to the host immune status. Virulence factors rely upon microbial attributes that mediate cell damage. While the activity of several Candida albicans hydrolytic enzymes is well characterized, the biological role of lipases is uncertain. In this report, we identified, isolated, and characterized a C. albicans 70 kDa lipase that exhibited maximal activity at physiological pH and temperature. We evaluated the ability of C. albicans lipase to interact with two types of mammalian host cells: macrophages, as crucial immune effector cells involved in fungal control, and hepatocytes, as examples of parenchymal cells compromised during fungal dissemination. Herein, we demonstrate for the first time that an extracellular lipase released by C. albicans directly induced cytotoxicity and promoted the deposition of lipid droplets in the cytoplasm of macrophages and hepatocytes

La virulence dépend de réactions opposées entre l’hôte et la pathogène et est liée de façon intrinsèque au statut immunologique de l’hôte. Les facteurs de virulence misent sur les attributs microbiens responsables du dommage cellulaire. Alors que l’activité de plusieurs enzymes hydrolytiques de Candida albicans est bien caractérisée, le rôle biologique des lipases est incertain. Dans cette étude, nous avons identifié, isolé et caractérisé une lipase de C. albicans de 70 kDa, qui présentait une activité maximale à température et pH physiologiques. Nous avons évalué la capacité de la lipase de C. albicans d’interagit avec deux types de cellules hôtes mammifères : les macrophages, une cellule immunitaire effectrice cruciale impliquée dans le contrôle de l’infection fongique, et les hépatocytes, un exemple de cellules parenchymateuses compromises lors de la dissémination fongique. Nous démontrons ici, pour la première fois, qu’une lipase extracellulaire secrétée par C. albicans induit directement la cytotoxicité et favorise le dépôt de gouttelettes lipidiques dans le cytoplasme des macrophages et des hépatocytes

Document Type: Research Article

Publication date: August 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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