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Revival of saprotrophic and mycorrhizal basidiomycete cultures after 20 years in cold storage in sterile water

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Vegetatively colonized agar cores of 69 basidiomycete fungus isolates (48 species in 30 genera and 17 families) were stored at 5 °C in tubes of sterile distilled water without manipulation for 20 years. These were represented by 34 isolates of saprotrophic fungi (29 species in 19 genera) and 35 isolates of mycorrhizal fungi (19 species in 11 genera). Viability was evaluated based on revived growth on agar media at room temperature. Fifty-seven of the 69 isolates (82.6%) grew vigorously when revived after storage for 20 years; of the 34 saprotrophic fungus isolates, 30 revived (88.2%); of the 35 mycorrhizal fungus isolates, 27 revived (77.1%). Thirteen isolates of Laccaria were all viable after 20 years, indicating cold storage in sterile water to be a good method for maintaining this important genus of mycorrhizal fungi. In general, however, mycorrhizal fungus species demonstrated lower viability than saprotrophic fungi.

Des échantillons d’agar colonisés de façon végétative comprenant 69 isolats de champignons basidiomycètes (48 espèces divisées en 30 genres et 17 familles) ont été entreposés à 5°C dans des tubes d’eau stérile distillée sans manipulation pendant 20 ans. Ceux-ci étaient représentés par 34 isolats de champignons saprotrophes (29 espèces divisées en 19 genres) et 35 isolats de mycorrhizes (19 espèces divisées en 11 genres). La viabilité a été évaluée par une reprise de la croissance sur agar à la température de la pièce. Cinquante-sept des 69 isolats (82.6 %) poussaient vigoureusement lorsque ravivés après un entreposage de 20 ans; des 34 isolats de champignons saprotrophes, 30 ont été ravivés (88.2 %); des 35 isolats de mycorrhizes, 27 ont été ravivés (77.1 %). Les 13 isolats de Laccaria étaient tous vivants après 20 ans, indiquant que l’entreposage au froid dans l’eau stérile est une bonne méthode de conservation de ce genre important de mycorrhizes. En général, cependant, les espèces de mycorrhizes démontraient une plus faible viabilité que les champignons saprotrophes.

Document Type: Research Article

Publication date: 2008-08-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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