Skip to main content

Oxylipin and mitochondrion probes to track yeast sexual cells

Buy Article:

$50.00 plus tax (Refund Policy)


When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests increased mitochondrial activity in asci, since 3-OH oxylipins are mitochondrially produced and it is known that Rh123 accumulates selectively in functional mitochondria that maintain a high transmembrane potential (ΔΨm). This increased activity may be necessary for the production and effective release of the many spores found in single-celled asci. These results may be useful in the rapid identification of asci and in yeast sexual spore mechanics, which may find application in yeast systematics as well as hydro-, aero-, and nano-technologies.

Lorsque des sondes de détection des oxylipines et des sondes mitochondriales, i.e., des anticorps fluorescents spécifiques aux acides gras 3-hydroylés (3-OH oxylipines) et la rhodamine 123 (Rh123), sont ajoutées à des levures, ces sondes s’accumulent principalement dans les cellules sexuelles (i.e., toutes deux associées aux ascospores) et non dans les cellules végétatives. Ceci suggère qu’il existe une augmentation de l’activité mitochondriale dans les asques puisque les 3-OH oxylipines sont produites dans la mitochondrie et que l’on sait que la Rh123 s’accumule sélectivement dans les mitochondries fonctionnelles pour maintenir un potentiel transmembranaire élevé (ΔΨm). Cette activité accrue peut être nécessaire à la production et la libération efficaces des nombreuses spores trouvées dans les asques à cellule unique. Ces résultats peuvent être utiles à l’identification rapide des asques et à la mécanique des spores sexuelles de la levure qui pourraient être appliqués en systématique de la levure ainsi qu’en hydro-, aéro- et nano-technologies.

Document Type: Research Article

Publication date: 2008-06-01

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free ContentFree content
  • Partial Free ContentPartial Free content
  • New ContentNew content
  • Open Access ContentOpen access content
  • Partial Open Access ContentPartial Open access content
  • Subscribed ContentSubscribed content
  • Partial Subscribed ContentPartial Subscribed content
  • Free Trial ContentFree trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more