Isolation and characterization of ZH14 with antiviral activity against Tobacco mosaic virus

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Abstract:

A large number of bacteria were isolated from plant samples and screened for antiviral activity against the Tobacco mosaic virus (TMV). The bacterium ZH14, which was isolated from Chinese Anxi oolong tea, secreted the antiviral substances, having 94.2% virus inhibition when the bacterial culture filtrate and TMV extract were mixed at a ratio of 1:1. The ZH14 strain is a gram-positive, spore-forming rod and has the ability to degrade ribonucleic acid. Based on its effectiveness on virus inhibition, ZH14 was selected for characterization and was identified as a strain of the Bacillus cereus group based on phenotypic tests and comparative analysis of its 16S rDNA sequence. At the same time, we determined the antiviral product of ZH14 as an extracellular protein with high molecular mass, having an optimum temperature of 15-60 °C and an optimum pH of 6-10. Hence, the ZH14 strain and its culture filtrate have potential application in controlling plant diseases caused by TMV.

Un grand nombre de bactéries ont été isolées d’échantillons végétaux et un criblage de leur activité antivirale envers le virus de la mosaïque du tabac (VMT) a été réalisé. Une bactérie, isolée du thé oolong Anxi chinois, ZH14, secrétait des substances antivirales capables d’inhiber le virus à 94,2 % lorsque le filtrat de la culture bactérienne et un extrait de VMT étaient mélangés dans un ratio de 1:1. La souche ZH14 était un bâtonnet gram-positif formant des spores ayant la propriété de dégrader l’acide ribonucléique. À cause de son efficacité à inhiber le virus, ZH14 a été caractérisée et a été identifiée comme souche appartenant au groupe Bacillus cereus grâce à des tests phénotypiques et à des analyses comparatives de la séquence de son ADNr 16S. Simultanément, nous avons déterminé que le composé antiviral de ZH14 était une protéine extracellulaire de haut poids moléculaire, active à température et pH optimaux de 15-60 °C et de 6 à 10 respectivement. Ainsi, la souche ZH14 et son filtrat de culture ont une application potentielle dans le contrôle des maladies des plantes causées par le VMT.

Document Type: Research Article

Publication date: June 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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