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Inhibition of Candida albicans extracellular enzyme activity by selected natural substances and their application in Candida infection

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Extracellular enzymes secreted by Candida albicans are claimed to be virulence factors responsible for penetration of the yeast into host cells. Substances able to inhibit lipolytic and proteinase activities of the fungus might be of therapeutic use in some pathologic conditions caused by C. albicans. In the present work, we have tested the influence of the flavonoid compounds apigenin and kaempferol, the indole alkaloid ibogaine, and the protoberberine alkaloid berberine on the in vitro enzyme activity of C. albicans. The substances showed complex suppressive effects concerning the processes of adherence to epithelial cells, secreted aspartyl proteinase activity, and the rate of cell wall protein glycosylation. Apigenin and kaempferol were administered in systemic C. albicans infection, demonstrating an increased number of survivors by kaempferol. The application of apigenin, kaempferol, ibogaine, and berberine in cutaneous infection suppressed the symptoms and accelerated elimination of the yeast from the site of inoculation.

On prétend que les enzymes extracellulaires sécrétées par Candida albicans sont les facteurs de virulence responsables de la pénétration de la levure dans les cellules hôtes. Les substances capables d’inhiber les activités lipolytiques et protéolytiques de champignons pourraient avoir une utilité thérapeutique dans certaines conditions pathologiques causées par C. albicans. Dans cette étude, nous avons testé l’influence de l’apigénine et du kaempférol, des composés flavonoïdes, de l’ibogaïne, un alcaloïde indolique, et de la berbérine, un alcaloïde de type protoberbérine, sur l’activité enzymatique de la levure in vitro. Les substances ont révélé des effets suppresseurs complexes sur les processus d’adhérence aux cellules épithéliales, l’activité aspartyl protéase sécrétée et le taux de glycosylation des protéines de la paroi cellulaire. L’apigénine et le kaempférol ont été administrés lors d’une infection systémique par C. albicans, le nombre de survivants étant augmenté avec le kaempférol. L’application des quatre substances lors d’une infection cutanée a supprimé les symptômes et a accéléré l’élimination du champignon du site d’inoculation.

Document Type: Research Article

Publication date: 2008-06-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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