Characterization of the Serratia marcescens SdeCDE multidrug efflux pump studied via gene knockout mutagenesis

$50.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Serratia marcescens is an important nosocomial agent having high antibiotic resistance. A major mechanism for S. marcescens antibiotic resistance is active efflux. To ascertain the substrate specificity of the S. marcescens SdeCDE efflux pump, we constructed pump gene deletion mutants. sdeCDE knockout strains showed no change in antibiotic susceptibility in comparison with the parental strains for any of the substrates, with the exception of novobiocin. In addition, novobiocin was the only antibiotic to be accumulated by sdeCDE-deficient strains. Based on the substrates used in our study, we conclude that SdeCDE is a Resistance-Nodulation-Cell Division family pump with limited substrate specificity.

Serratia marcescens est un agent nosocomial important hautement résistant aux antibiotiques. L’efflux actif est le principal mécanisme responsable de la résistance aux antibiotiques de S. marcescens. Afin d’étudier la spécificité de substrat de la pompe d’efflux SdeCDE de S. marcescens, nous avons construit des mutants de délétion du gène codant la pompe. La sensibilité aux antibiotiques était inchangée chez les souches ayant subi une ablation génique de sdeCDE comparativement aux lignées parentales et ce, pour tous les substrats à l’exception de la novobiocine. De plus, la novobiocine était le seul antibiotique à s’accumuler dans les souches déficientes en sdeCDE. En se fondant sur les substrats utilisés dans notre étude, nous concluons que SdeCDE est un membre de la famille des pompes dites de « Resistance-Nodulation-Cell Division » possédant une spécificité de substrat limitée.

Document Type: Research Article

Publication date: May 1, 2008

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Sample Issue
  • Reprints & Permissions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more