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Seasonal variation in communities of ammonia-oxidizing bacteria based on polymerase chain reaction - denaturing gradient gel electrophoresis in a biofilm reactor for drinking water pretreatment

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Abstract:

The diversity and variation of total and active ammonia-oxidizing bacteria in a full-scale aerated submerged biofilm reactor for drinking water pretreatment were characterized by clone libraries and denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA and its gene during a whole year. Sequences obtained from clone libraries affiliated with the Nitrosomonas oligotropha lineage and the Nitrosomonas communis lineage. An uncultured subgroup of Nitrosomonas communis lineage was also detected. Seasonal variations in both total and active ammonia-oxidizing bacteria communities were observed in the DGGE profiles, but an RNA-based analysis reflected more obvious dynamic changes in ammonia-oxidizer community than a DNA-based approach. Statistical study based on canonical correspondence analysis showed that a community shift of active ammonia oxidizers was significantly influenced by temperature and pH, but no significant correlation was found between environmental variables and total ammonia-oxidizer community shift.

Les auteurs ont caractérisé au cours d’une année complète la diversité et la variation de populations de bactéries totales et de bactéries actives oxydant l’ammoniac, présentes dans un réacteur aéré à biofilm submergé utilisé dans le prétraitement de l’eau de consommation, à l’aide de banques de clones et d’électrophorèse sur gel en gradient dénaturant (DGGE) de l’ARNr 16S et de son gène. Les séquences obtenues des banques de clones correspondaient aux lignées Nitrosomonas oligotropha et Nitrosomonas communis. Un sous-groupe non cultivé de Nitrosomonas communis a aussi été détecté. Des variations saisonnières de communautés de bactéries totales et actives oxydant l’ammoniac ont été observées à l’aide des profils de DGGE, mais les analyses de l’ARN reflétaient des changements dynamiques de la population oxydant l’ammoniac plus évidents que les analyses d’ADN. Une étude statistique basée sur des analyses de correspondance canonique a démontré que la modulation de la communauté active oxydant l’ammoniac était influencée de façon significative par la température et le pH, mais aucune corrélation significative n’a été trouvée entre les variables environnementales et la modulation de la communauté totale oxydant l’ammoniac.

Document Type: Research Article

Publication date: May 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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