attG and attC mutations of Agrobacterium tumefaciens are dominant negative mutations that block attachment and virulence

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Abstract:

The cryptic plasmid (pAT) of Agrobacterium tumefaciens was not required for virulence or attachment to plant surfaces. However, mutations in the attC and attG genes located on pAT caused the bacteria to become avirulent and nonattaching on tomato, carrot, and Bryophyllum daigremontiana. This was the case whether the mutation was in the copy of the genes located on pAT or whether it was carried in a second copy of the attA-G operon located on a plasmid in cells that contained a wild-type copy of pAT. Thus attC and attG mutations are dominant negative mutations. The mechanism by which these mutations block attachment and virulence is unknown.

Le plasmide cryptique pAT de Agrobacterium tumefaciens n’était par requis à sa virulence ou à son adhérence aux surfaces des plantes. Cependant, des mutations au sein des gènes attC et attG localisés sur pAT rendaient les bactéries non virulentes et non adhérentes sur la tomate, la carotte et sur Bryophyllum daigremontiana. Ceci survenait, peu importe si la mutation était présente dans la copie des gènes localisée sur pAT ou sur la deuxième copie de l’opéron attA-G localisée sur un plasmide chez les cellules qui contiennent une copie sauvage de pAT. Ainsi, les mutations attC et attG sont des mutations dominantes négatives. Le mécanisme par lequel ces mutations bloquent l’adhérence et la virulence est inconnu.

Document Type: Research Article

Publication date: April 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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