Differentiation of Sclerotinia minor depends on thiol redox state and oxidative stress

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Abstract:

Sclerotial differentiation in Sclerotinia minor is associated with oxidative stress and thiol redox state. The significance of oxidative stress to sclerotial differentiation was revealed by the higher oxidative stress of S.minor compared with a nonsclerotiogenic counterpart. The effect of thiol redox state on sclerotial differentiation was shown by the antioxidant action of the thiol (-SH) group of N-acetylcysteine and cysteine and by an unknown (not antioxidant) role of glutathione (GSH) on S.minor. The nonantioxidant role of GSH was indicated by the differentiation-inhibiting and differentiation-noninhibiting actions of the GSH biosynthesis inhibitor -buthionine-S,R-sulfoximine and the GSH biosynthesis inducer -2-oxo-thiazolidine-4-carboxylate, respectively, and by the increase of oxidative stress they caused during the transition from the undifferentiated to differentiated state of S.minor. Moreover, N-acetylcysteine can be used as a potent nontoxic fungicide against this phytopathogenic fungus by acting as a growth-inhibiting cytotoxic oxidant and by sustaining the fungus in the undifferentiated hyphal stage, which is vulnerable to degradation by soil microorganisms.

La différenciation en sclérote de Sclerotinia minor est associée au stress oxydatif et à l’état redox des thiols. La première association a été mise en évidence par l’observation d’un stress oxydatif plus élevé chez S.minor comparativement à sa contrepartie non-sclérotinogène. La deuxième association a été démontrée par l’activité anti-oxydante des groupes -SH de la N-acétylcystéine et de la cystéine, ainsi que par une activité indéterminée (non anti-oxydante) du glutathion lors de la différenciation en sclérote de S.minor. Cette dernière a été démontrée par les activités respectivement inhibitrice et non inhibitrice de la différenciation du -buthionine-S,R-sulfoximine, un inhibiteur de la biosynthèse du GSH, et du -2-oxo-thiazolidine-4-carboxylate, un inducteur de la biosynthèse du GSH, ainsi que par l’augmentation du stress oxydatif qu’ils induisent lors de la transition de l’état non différencié vers l’état différencié de S.minor. Qui plus est, le N-acétylcystéine peut être utilisée comme fongicide puissant non toxique pour l’hôte, agissant contre ce champignon phytopathogène comme un oxydant cytotoxique qui inhibe la croissance et qui maintient le champignon dans un état d’hyphe non différenciée, vulnérable à la dégradation par les microorganismes du sol.

Document Type: Research Article

Publication date: January 1, 2008

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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