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Pathogenic potential of a collagenase gene from Aeromonas veronii

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The role of collagenase as a mechanism of bacterial pathogenicity in some pathogenic bacteria has been reported. The information on the role of collagenase in Aeromonas spp. pathogenesis is scant. In the present study, a mutant Aeromonas veronii RY001 that is deficient in the putative collagenase gene acg was constructed and compared with the wild-type strain for virulence factors. Bacterial cells and cell-free extracellular products of the mutant had significantly less collagenolytic activity, but there were not significant differences in caseinolytic, gelatinolytic, and elastolytic activities. Adhesion and invasion abilities of the mutant strain on epithelioma papillosum of carp cells was only 56% of that of the wild-type strain, and the cytotoxicity of the mutant strain to epithelioma papillosum of carp cells was only 42% of that of the wild-type strain. The LD50 values of the wild-type strain were determined as 1.6 × 106 and 3.5 × 105 cfu in goldfish and mice, respectively, whereas the mutant RY001 strain showed slightly higher values (i.e., 2.8 × 106 and 1.4 × 106 cfu in goldfish and mice, respectively). These results indicated the involvement of the collagenase gene in the pathogenesis of A. veronii.

Le rôle de la collagénase dans la pathogénicité bactérienne chez certaines bactéries pathogènes a été rapporté. Les informations sur le rôle de la collagénase dans la pathogenèse liée à Aeromonas sont limitées. Dans cette étude, le mutant Aeromonas veronii RY001, déficient quant au gène présumé de la collagénase (soit acg), a été construit et comparé à la souche sauvage relativement aux facteurs de virulence. Les cellules bactériennes et les produits acellulaires extracellulaires du mutant possédaient une activité collagénolytique significativement moindre, mais les activités caséinolytiques, gélatinolytiques et élastinolytiques n’étaient pas significativement différentes. L’adhérence de la souche mutante et sa capacité d’invasion des cellules de carpes EPC (Epithelioma papillosum cyprinii) n’étaient que de 56 % des valeurs observées chez la souche sauvage, alors que la cytotoxicité de la souche mutante pour les cellules EPC n’était que de 42 % de celle observée avec la souche sauvage. Les valeurs de LD50 de la souche sauvage étaient de 1,6 × 106 cfu et de 3,5 × 105 cfu, respectivement chez le poisson rouge et la souris, alors que le mutant RY001 affichait des valeurs légèrement plus élevées, soit 2,8 × 106 et 1,4 × 106 cfu chez le poisson rouge et la souris, respectivement. Ces résultats suggèrent que le gène de la collagénase soit impliqué dans la pathogenèse associée à A. veronii.

Document Type: Research Article

Publication date: 2008-01-01

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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