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The antioxidants dimethylsulfoxide and dimethylthiourea affect the immediate adhesion responses of larval haemocytes from 3 lepidopteran insect species

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Abstract:

Antioxidants, dimethylsulfoxide (DMSO) and dimethylthiourea (DMTU), at concentrations not affecting the viability of blood cells (haemocytes) from the larval stage of 3 lepidopteran insects — Galleria mellonella, Lymantria dispar, and Malacosoma disstria — differed in their influence on the innate binding of haemocytes to glass, bacteria to haemocytes, and on humoral responses to alien materials. In vitro DMSO had little effect, whereas DMTU substantially impaired the adhesion of the haemocyte types, the plasmatocytes and granular cells, to slides as well as the attachment of Bacillus subtilis to these haemocytes. Although both antioxidants increased lysozyme and phenoloxidase activities, there was no correlation of enzyme activity and haemocyte adhesion responses, possibly reflecting sequestered radicals. Nitric oxide and hydroxyl radicals offset the DMTU effect. In the absence of antioxidants, inactivate protein kinases A (PKA) and C (PKC) enhanced haemocyte aggregation. In general, DMSO, as opposed to DMTU, did not alter the effects of PKA and PKC activators and inhibitors on haemocyte aggregation or of PKC and PKA activities. High concentrations of DMSO and all levels of DMTU, although inhibiting PKA and PKC, inhibited haemocyte adhesion to slides. Comparable results occurred for DMTU-treated haemocytes incubated with B.subtilis. In vivo DMSO, unlike DMTU, did not impair plasmatocyte or granular cell responses to foreign materials, including bacterial removal from the haemolymph and nodulation.

Des antioxydants, le diméthylsulfoxyde (DMSO) et la diméthylthiourée (DMTU), utilisés à des concentrations n’affectant pas la viabilité des cellules sanguines (hémocytes) des larves de trois insectes lépidoptères— Galleria mellonella, Lymantria dispar et Malacosoma disstria— diffèrent quant à leur influence sur l’adhérence innée des hémocytes au verre, l’adhérence des bactéries aux hémocytes, ainsi que sur les réponses humorales face aux agents étrangers. In vitro, le DMSO a peu d’effet alors que la DMTU empêche de façon importante l’adhérence de deux types d’hémocytes, les plasmatocytes et les cellules granulaires, aux lames et l’attachement de Bacillus subtilis à ces hémocytes. Quoique les deux antioxydants augmentent l’activité lysozyme et phénoloxydase, il n’y avait pas de corrélation entre l’activité enzymatique et l’adhérence des hémocytes, reflétant possiblement la séquestration de radicaux. L’oxyde nitrique et les radicaux hydroxyles compensent les effets de la DMTU. En absence d’antioxydants, l’inactivation des protéine kinases A (PKA) et C (PKC) augmentait l’agrégation des hémocytes. En général, le DMSO, contrairement à la DMTU, ne modifiait pas les effets d’activateurs ou d’inhibiteurs de la PKA et de la PKC sur l’agrégation des hémocytes ou sur l’activité de la PKA et de la PKC. De fortes concentrations de DMSO et toutes les concentrations de DMTU, même si elles inhibaient la PKA et la PKC, inhibaient l’adhésion des hémocytes aux lames. Des résultats comparables ont été obtenus avec les hémocytes traités à la DMTU incubés avec B.subtilis. In vivo, le DMSO, contrairement à la DMTU, n’interfèrait pas avec les réponses des plasmatocytes et des cellules granulaires aux agents étrangers incluant l’enlèvement des bactéries de l’hémolymphe et la nodulation.

Document Type: Research Article

Publication date: December 1, 2007

More about this publication?
  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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