ACC deaminase from plant growth-promoting bacteria affects crown gall development

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In addition to the well-known roles of indoleacetic acid and cytokinin in crown gall formation, the plant hormone ethylene also plays an important role in this process. Many plant growth-promoting bacteria (PGPB) encode the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which can degrade ACC, the immediate precursor of ethylene in plants, to α-ketobutyrate and ammonia and thereby lower plant ethylene levels. To study the effect of ACC deaminase on crown gall development, an ACC deaminase gene from the PGPB Pseudomonas putida UW4 was introduced into Agrobacterium tumefaciens C58, so that the effect of ACC deaminase activity on tumour formation in tomato and castor bean plants could be assessed. Plants were also coinoculated with A.tumefaciens C58 and P.putida UW4 or P.putida UW4-acdS (an ACC deaminase minus mutant strain). In both types of experiments, it was observed that the presence of ACC deaminase generally inhibited tumour development on both tomato and castor bean plants.

En plus de l’acide indoleacétique et de la cytokinine, dont les rôles dans la formation de la maladie du collet (le crown gall) sont bien connus, l’éthylène, une hormone végétale, joue aussi un rôle important dans ce processus. Plusieurs bactéries promotrices de la croissance des plantes (PGPB) expriment l’enzyme 1-aminocyclopropane-1-carboxylate (ACC) désaminase qui peut dégrader l’ACC, le précurseur immédiat de l’éthylène chez les plantes, en α-cétobutyrate et en ammoniaque, abaissant ainsi les niveaux d’éthylène chez la plante. Afin d’étudier les effets de l’ACC désaminase sur le développement de la maladie du collet, le gène codant l’ACC désaminase chez la PGPB Pseudomonas putida UW4 a été introduit dans Agrobacterium tumefaciens C58 afin que l’effet de l’activité de l’ACC désaminase sur la formation de la tumeur chez des plants de tomates et de fèves de ricin puisse être étudié. Les plants ont aussi été co-inoculés avec A.tumefaciens C58 et P.putida UW4 ou P.putida UW4-acdS, (une souche mutante sans ACC désaminase). Dans les deux types d’expériences, l’on a observé que la présence d’ACC désaminase inhibait généralement le développement de la tumeur chez les plants de tomates et de graines de ricin.

Document Type: Research Article

Publication date: December 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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