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Possible requirement of intercellular adhesion molecule-1 for invasion of gingival epithelial cells by Treponema medium

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Abstract:

Oral treponemes are members of the spirochete family of bacteria associated with periodontal diseases. In the present study, we demonstrate that intercellular adhesion molecule-1 (ICAM-1) on human gingival epithelial cells (HGEC) contributed to the invasion of Treponema medium, a medium-sized oral Treponema, into those cells. The quantity of T.medium in HGEC was found to peak at 2h after inoculation and then decreased gradually. Immunofluorescence microscopy findings showed that the bacteria were colocalized with ICAM-1 on HGEC. Furthermore, knockdown of ICAM-1 in HGEC resulted in inhibition of T.medium invasion by RNA interference, whereas that of Toll-like receptor 2 did not. These results suggest that ICAM-1 may be required for the invasion of T.medium into HGEC, and they indicate that the molecule plays a principal role in the primary stages of the development and progression of chronic periodontitis.

Les tréponèmes oraux sont des membres de la famille des bactéries spirochètes associées aux maladies parodontales. Dans cette étude, nous avons démontré que la molécule d’adhésion intercellulaire ICAM-1 présente à la surface des cellules épithéliales gingivales humaines (HGEC) contribuait à l’invasion de ces cellules par Treponema medium, un Treponema oral de taille moyenne. La quantité de T. medium dans les HGEC atteignait un pic deux heures après l’inoculation et diminuait alors graduellement. Des résultats en microscopie en immunofluorescence ont montré que la localisation des bactéries coïncidait avec celle de la ICAM-1 sur les HGEC. Qui plus est, l’inactivation de la ICAM-1 par interférence à l’ARN chez les HGEC résultait en une inhibition de l’invasion par T. medium, alors que l’inactivation de récepteur «Toll-like» TLR2 n’y parvenait pas. Ces résultats suggèrent que la ICAM-1 puisse être requise pour l’invasion des HGEC par T. medium et indiquent que la molécule joue un rôle clé dans les premières étapes du développement et de la progression de la périodontite chronique.

Document Type: Research Article

Publication date: November 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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