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Yersinia genome diversity disclosed by Yersinia pestis genome-wide DNA microarray

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Abstract:

The genus Yersinia includes 11 species, 3 of which (Y. pestis, Y.pseudotuberculosis, and Y. enterocolitica) are pathogenic for humans. The remaining 8 species (Y.frederiksenii, Y. intermedia, Y. kristensenii, Y. bercovieri, Y.mollaretii, Y. rohdei, Y. ruckeri, and Y.aldovae) are merely opportunistic pathogens found mostly in the environment. In this work, the genomic differences among Yersinia were determined using a Y.pestis-specific DNA microarray. The results revealed 292 chromosomal genes that were shared by all Yersinia species tested, constituting the conserved gene pool of the genus Yersinia. Hierarchical clustering analysis of the microarray data revealed the genetic relationships among all 11 species in this genus. The microarray analysis in conjunction with PCR screening greatly reduced the number of chromosomal genes (32) specific for Y.pestis to 16 genes and uncovered a high level of genomic plasticity within Y.pseudotuberculosis, indicating that its different serotypes have undergone an extensively parallel loss or acquisition of genetic content, which is likely to be important for its adaptation to changes in environmental niches.

Le genre Yersinia comprend 11 espèces dont 3 sont pathogènes pour l’humain: Y.pestis, Y.pseudotuberculosis et Y.enterocolitica. Les 8 autres espèces (Y.frederiksenii, Y.intermedia, Y.kristensenii, Y.bercovieri, Y.mollaretii, Y.rohdei, Y.ruckeri et Y.aldovae) sont des pathogènes essentiellement opportunistes trouvés principalement dans l’environnement. Dans ce travail, les différences génomiques entre les espèces du genre Yersinia ont été déterminées à l’aide de puces à ADN spécifiques à l'Y.pestis. Les résultats ont révélé la présence de 292 gènes chromosomiques qui sont partagés par toutes les espèces de Yersinia testées, constituant ainsi un pool de gènes conservés du genre Yersinia. Une analyse hiérarchique des grappes obtenues des résultats des puces à ADN a révélé la présence d’une relation génétique parmi les 11 espèces de ce genre. L’analyse des puces à ADN en combinaison avec un criblage par PCR a grandement réduit le nombre de gènes chromosomiques (32) spécifiques à l'Y.pestis à 16 gènes et a révélé un haut degré de plasticité génomique chez Y.pseudotuberculosis, indiquant que ces différents sérotypes ont subi des pertes ou des acquisitions parallèles de contenu génétique, ce qui est probablement important pour son adaptation aux changements de ses niches environnementales.

Document Type: Research Article

Publication date: November 1, 2007

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  • Published since 1954, this monthly journal contains new research in the field of microbiology including applied microbiology and biotechnology; microbial structure and function; fungi and other eucaryotic protists; infection and immunity; microbial ecology; physiology, metabolism and enzymology; and virology, genetics, and molecular biology. It also publishes review articles and notes on an occasional basis, contributed by recognized scientists worldwide.
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